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Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri T-DNA gene integration
Hairy root cultures of Catharanthus roseus var. Prabal were established by infecting the leaves with Agrobacterium rhizogenes agropine-type A4 strain. Two hundred and fifty independent root clonesExpand
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Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1 (Cas12a)
  • D. Singh, J. Mallon, +5 authors T. Ha
  • Chemistry, Medicine
  • Proceedings of the National Academy of Sciences
  • 7 May 2018
Significance Adaptive immunity against foreign DNA elements in microbes, conferred by their CRISPR systems, has been repurposed for wide-ranging genome-engineering applications. The CRISPR-Cas9Expand
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Expression of terpenoid indole alkaloid biosynthetic pathway genes corresponds to accumulation of related alkaloids in Catharanthus roseus (L.) G. Don
Madagascar periwinkle, Catharanthus roseus (L.) G. Don, a medicinally important plant, produces anticancer dimeric alkaloids, vinblastine and vincristine, in the leaves and accumulatesExpand
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Assessment and rationalization of genetic diversity of Papua New Guinea taro (Colocasia esculenta) using SSR DNA fingerprinting
Taro (Colocasia esculenta) accessions were collected from 15 provinces of Papua New Guinea (PNG). The collection, totalling 859 accessions was collated for characterization and a core collection ofExpand
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Mechanisms of improved specificity of engineered Cas9s revealed by single-molecule FRET analysis
  • D. Singh, Y. Wang, +6 authors T. Ha
  • Chemistry, Medicine
  • Nature Structural & Molecular Biology
  • 1 April 2018
Cas9 (from Streptococcus pyogenes) in complex with a guide RNA targets complementary DNA for cleavage. Here, we developed a single-molecule FRET analysis to study the mechanisms of specificityExpand
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Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9
Binding specificity of Cas9-guide RNA complexes to DNA is important for genome engineering applications, but how mismatches influence target recognition and rejection kinetics is not well understood.Expand
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Real-time observation of DNA target interrogation and product release by the RNA-guided endonuclease CRISPR Cpf1
CRISPR-Cas9, which imparts adaptive immunity against foreign genomic invaders in certain prokaryotes, has been repurposed for genome-engineering applications. More recently, another RNA-guided CRISPRExpand
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Single molecule analysis of effects of non-canonical guide RNAs and specificity-enhancing mutations on Cas9-induced DNA unwinding
Cas9 has made a wide range of genome engineering applications possible. However, its specificity continues to be a challenge. Non-canonical gRNAs and new engineered variants of Cas9 have beenExpand
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Alkaloid profiles of the Indian land races of the opiumpoppy Papaver somniferum L.
The capsule husks of a set of 115 Indian land races of opium poppy Papaver somniferum L. obtained over two cropping seasons were screened for the percent contents of papaverine, reticuline,Expand
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Transcript profiling of terpenoid indole alkaloid pathway genes and regulators reveals strong expression of repressors in Catharanthus roseus cell cultures
The understanding of the complexities and molecular events regulating genes and the activators involved in terpenoid indole alkaloid (TIA) metabolism is known to a certain extent in cell cultures ofExpand
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