Purification and characterisation of an antifreeze protein from Forsythia suspensa (L.).
- D. Simpson, M. Smallwood, S. Twigg, C. J. Doucet, Joe Ross, D. Bowles
- BiologyCryobiology
- 1 October 2005
Molecular and morphological discrimination of stipitate hydnoids in the genera Hydnellum and Phellodon.
- D. Parfitt, A. Martyn Ainsworth, D. Simpson, H. Rogers, L. Boddy
- Environmental Science, BiologyMycological Research
- 1 July 2007
Nitrification by plants that also fix nitrogen
- C. Hipkin, D. Simpson, S. J. Wainwright, M. Salem
- Environmental ScienceNature
- 1 July 2004
It is shown how certain legumes that accumulate the toxin, 3-nitropropionic acid, generate oxidized inorganic nitrogen in their shoots, which is returned to the soil in their litter in a previously undescribed feature of this biogeochemical cycle.
3-nitropropionic acid oxidase from horseshoe vetch (Hippocrepis comosa): a novel plant enzyme.
- C. Hipkin, M. Salem, D. Simpson, S. J. Wainwright
- BiologyBiochemical Journal
- 1 June 1999
A novel enzyme that catalyses the oxygen-dependent oxidation of 3-nitropropionic acid (3NPA) to malonate semialdehyde, nitrate, nitrite and H2O2 has been purified from leaf extracts of the horseshoe…
Transformation of Acinetobacter baylyi in non-sterile soil using recombinant plant nuclear DNA.
- D. Simpson, J. Fry, H. Rogers, M. Day
- BiologyEnvironmental Biosafety Research
- 2007
To provide estimates of horizontal gene transfer from transgenic crops to indigenous soil bacteria, transformation frequencies were obtained for naturally transformable Acinetobacter baylyi BD413…
Influence of flanking homology and insert size on the transformation frequency of Acinetobacter baylyi BD413.
- D. Simpson, Lisa F. Dawson, J. Fry, H. Rogers, M. Day
- BiologyEnvironmental Biosafety Research
- 2007
It is shown that the ratio of flanking homology to insert size and not the total size of donor DNA is the most important variable determining transformation frequency.