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Expression and Immunogenicity of Mycoplasma hyopneumoniae Heat Shock Protein Antigen P42 by DNA Vaccination
ABSTRACT Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia, a chronic nonfatal disease affecting pigs of all ages. The goal of this study was to design DNA vaccines byExpand
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Recombinant Candida utilis for the production of biotin
Biotin is an important nutritional supplement but is difficult to manufacture effectively. Here we present a trial of biotin production using the food yeast Candida utilis. In this system, we clonedExpand
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Mycoplasmas regulate the expression of heat-shock protein genes through CIRCE-HrcA interactions.
Mycoplasmas in general are rarely exposed to severe environmental changes except during its colonization and infection processes. Genomic analysis indicates that Mycoplasma hyopneumoniae possessesExpand
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Differential inhibition of respiration and its dependent H+ extrusion by fluorescamine in rat liver mitochondria.
Abstract Rat liver mitochondria were treated with varying amounts of fluorescamine ranging from 0 to 30 nmol/mg of protein. The biochemical activities of the modified mitochondria were analyzed. ItExpand
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Determination of biotin concentration by a competitive enzyme-linked immunosorbent assay (ELISA) method.
A method based on competitive enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of biotin concentrations which takes advantage of the extraordinarily high affinity betweenExpand
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Lysozyme association with nucleic acids.
Lysozyme is well known for the ability to hydrolyze the cell wall of bacteria. Based on the similarity of structure between lysozyme and histones as seen from the results of X-ray crystal structureExpand
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Effects of fluorescamine modification on light-induced H+-movement in reconstituted purple membrane of halobacteria.
Abstract The reconstituted purple membrane of halobacterium halobium was treated with amine-specific reagents, fluorescamine, NBD-C1, DNFB and pyridoxal phosphate. Only the fluorescamine modificationExpand
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Recombinant Truncated Flagellin of Burkholderia pseudomallei as a Molecular Probe for Diagnosis of Melioidosis
ABSTRACT Current serological tests for melioidosis, using impure or uncharacterized cell antigens from Burkholderia pseudomallei, have problems in detection sensitivity and specificity. Therefore, weExpand
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A single Ser-180 mutation desensitizes feedback inhibition of the phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthetase in Escherichia coli.
The Escherichia coli phenylalanine-sensitive DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate) synthetase (aroG product) is one of the DAHP synthetase isozymes that catalyze the first committed stepExpand
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Proteomic comparative analysis of pathogenic strain 232 and avirulent strain J of Mycoplasma hyopneumoniae
Mycoplasma hyopneumoniae is an important pathogen of pigs causing enzootic pneumonia of swine. The pathogen remains largely enigmatic as far as the host-pathogen interactions are concerned. In theExpand
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