Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis
- Y. Ionov, María Angeles Peinado, S. Malkhosyan, D. Shibata, M. Perucho
- Biology, MedicineNature
- 10 June 1993
It is shown that 12 per cent of colorectal carcinomas carry somatic deletions in poly(dA . dT) sequences and other simple repeats, and it is concluded that these mutations reflect a previously undescribed form of carcinogenesis in the colon mediated by a mutation in a DNA replication factor resulting in reduced fidelity for replication or repair (a 'mutator mutation').
MethyLight: a high-throughput assay to measure DNA methylation.
- C. Eads, K. Danenberg, P. Laird
- BiologyNucleic Acids Research
- 15 April 2000
A high-throughput quantitative methylation assay that utilizes fluorescence-based real-time PCR (TaqMan) technology that requires no further manipulations after the PCR step is described, and MethyLight is a highly sensitive assay, capable of detecting methylated alleles in the presence of a 10,000-fold excess of unmethylated allele.
Most human carcinomas of the exocrine pancreas contain mutant c-K-ras genes
- C. Almoguera, D. Shibata, K. Forrester, John Martin, N. Arnheim, M. Perucho
- Biology, MedicineCell
- 20 May 1988
A Big Bang model of human colorectal tumor growth
- A. Sottoriva, Haeyoun Kang, C. Curtis
- BiologyNature Genetics
- 9 February 2015
A 'Big Bang' model is presented, whereby tumors grow predominantly as a single expansion producing numerous intermixed subclones that are not subject to stringent selection and where both public and most detectable private alterations arise early during growth.
Variable Clonal Repopulation Dynamics Influence Chemotherapy Response in Colorectal Cancer
- A. Krešo, C. O'Brien, J. Dick
- BiologyScience
- 1 February 2013
Combining DNA copy number alteration (CNA) profiling, sequencing, and lentiviral lineage tracking, this work followed the repopulation dynamics of 150 single lentivirus-marked lineages from 10 human colorectal cancers through serial xenograft passages in mice, showing that clones remained stable upon serial transplantation and Chemotherapy promoted the dominance of previously minor or dormant lineages.
Localization of a susceptibility locus for Peutz-Jeghers syndrome to 19p using comparative genomic hybridization and targeted linkage analysis
- A. Hemminki, I. Tomlinson, L. Aaltonen
- MedicineNature Genetics
- 1997
A search for a putative tumour suppressor locus was made using comparative genomic hybridization of Peutz-Jeghers polyps, combined with loss of heterozygosity (LOH), and molecular evidence of malignant potential in hamartomas is provided.
Epstein-Barr virus-associated gastric adenocarcinoma.
- D. Shibata, L. Weiss
- Medicine, BiologyAmerican Journal of Pathology
- 1 April 1992
The authors investigated the possibility that EBV may be present not only in the rare gastric cancers that resemble nasopharyngeal lymphoepithelioma, but also in typical gastric adenocarcinoma, and found some cases of gastric carcinoma are EBV-associated.
Deficiencies in mouse Myh and Ogg1 result in tumor predisposition and G to T mutations in codon 12 of the K-ras oncogene in lung tumors.
- Yali Xie, Hanjing Yang, Jeffrey H. Miller
- BiologyCancer Research
- 1 May 2004
It is demonstrated that deficiencies in Myh and Ogg1 predispose 65.7% of mice to tumors, predominantly lung and ovarian tumors, and lymphomas, and codon 12 of K-ras is likely to be an important downstream target in lung tumorigenesis.
BCL2 translocation frequency rises with age in humans.
- Y. Liu, A. Hernández, D. Shibata, G. Cortopassi
- Medicine, BiologyProceedings of the National Academy of Sciences…
- 13 September 1994
Findings demonstrate that clones harboring the oncogenic t(14;18) chromosomal translocation are commonly present in normal humans, that such clones are long-lived, and that they rise in frequency with age.
The DCC protein and prognosis in colorectal cancer.
- D. Shibata, M. Reale, I. Summerhayes
- MedicineNew England Journal of Medicine
- 5 December 1996
In stage II colorectal carcinomas, the absence of DCC identifies a subgroup of patients with lesions that behave like stage III cancers, which may have therapeutic implications in this group of patients.
...
...