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Methods for qPCR gene expression profiling applied to 1440 lymphoblastoid single cells
Highlights ► Microfluidic arrays enable analysis of 96 qPCR assays on 1440 single cells. ► Detailed methods on obtaining qPCR data and performing preliminary data processing. ► Data from sufficientExpand
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Trypanosoma brucei: chemical evidence that cathepsin L is essential for survival and a relevant drug target.
The protozoan parasite causing human African trypanosomiasis, Trypanosoma brucei, displays cysteine peptidase activity, the chemical inhibition of which is lethal to the parasite. This activityExpand
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Single-cell gene expression analysis reveals genetic associations masked in whole-tissue experiments.
Gene expression in multiple individual cells from a tissue or culture sample varies according to cell-cycle, genetic, epigenetic and stochastic differences between the cells. However, single-cellExpand
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Corticosteroids, eosinophils and bronchial epithelial cells: new insights into the resolution of inflammation in asthma.
Anti-inflammatory therapy in asthma is reliant on corticosteroids, particularly in their inhaled form. However, steroids are rather non-specific in their actions and they also raise concernsExpand
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Giardia secretome highlights secreted tenascins as a key component of pathogenesis
Abstract Background Giardia is a protozoan parasite of public health relevance that causes gastroenteritis in a wide range of hosts. Two genetically distinct lineages (assemblages A and B) areExpand
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Effectiveness of Common Household Cleaning Agents in Reducing the Viability of Human Influenza A/H1N1
Background In the event of an influenza pandemic, the majority of people infected will be nursed at home. It is therefore important to determine simple methods for limiting the spread of the virusExpand
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Human alveolar epithelial cells engulf apoptotic eosinophils by means of integrin- and phosphatidylserine receptor-dependent mechanisms: a process upregulated by dexamethasone.
BACKGROUND We previously demonstrated receptor-mediated apoptotic-eosinophil engulfment by human small airway epithelial cells, which may represent a potentially important mechanism in the resolutionExpand
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H‐ficolin binds Aspergillus fumigatus leading to activation of the lectin complement pathway and modulation of lung epithelial immune responses
Aspergillus fumigatus is an opportunistic fungal pathogen that typically infects the lungs of immunocompromised patients leading to a high mortality. H‐Ficolin, an innate immune opsonin, is producedExpand
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P2Y6 receptor inhibition perturbs CCL2-evoked signalling in human monocytic and peripheral blood mononuclear cells
ABSTRACT The chemokine CCL2 serves to target circulating monocytes and other leukocytes to tissue during innate immune responses, and modulates the progression of chronic inflammatory disease throughExpand
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Resting and cytokine-stimulated human small airway epithelial cells recognize and engulf apoptotic eosinophils.
Eosinophils, which are prominent cells in asthmatic inflammation, undergo apoptosis and are recognized and engulfed by phagocytic macrophages in vitro. We have examined the ability of human smallExpand
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