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Use of polymerase chain reaction (PCR) to study transmission of banana bunchy top virus by the banana aphid (Pentalonia nigronervosa)
Summary Banana bunchy top virus (BBTV) is a ssDNA virus transmitted by the banana aphid, (Pentalonia nigronervosa). A polymerase chain reaction (PCR) assay was used to study BBTV transmission… Expand
Characterization of a virus infecting Citrus volkameriana with citrus leprosis-like symptoms.
A Citrus volkameriana tree displaying symptoms similar to citrus leprosis on its leaves and bark was found in Hawaii. Citrus leprosis virus C (CiLV-C)-specific detection assays, however, were… Expand
Use of a Tissue Blotting Immunoassay to Examine the Distribution of Pineapple Closterovirus in Hawaii.
Specific monoclonal antibodies made to a pineapple closterovirus (PCV) were used in a tissue blotting immunoassay (TBIA) for the detection of PCV in pineapple. More than 2,000 samples were tested in… Expand
Diversity and Mealybug Transmissibility of Ampeloviruses in Pineapple.
Mealybug wilt of pineapple (MWP) is one of the most destructive diseases of pineapple (Ananas comosus) worldwide. At least one Ampelovirus species, Pineapple mealybug wilt associated virus-2… Expand
Transmission of Pineapple Mealybug Wilt-Associated Virus by Two Species of Mealybug (Dysmicoccus spp.).
ABSTRACT Closterovirus-like particles associated with mealybug wilt of pineapple were acquired and transmitted by the pink pineapple mealybug, Dysmicoccus brevipes, and the gray pineapple mealybug,… Expand
Genetic diversity and evidence for recent modular recombination in Hawaiian Citrus tristeza virus
- M. Melzer, W. Borth, D. Sether, S. Ferreira, D. Gonsalves, J. Hu
- Biology, Medicine
- Virus Genes
- 1 February 2010
Abstract The Hawaiian Islands are home to a widespread and diverse population of Citrus tristeza virus (CTV), an economically important pathogen of citrus. In this study, we quantified the genetic… Expand
Complete nucleotide sequence and genome organization of pineapple mealybug wilt-associated virus-1
- M. Melzer, D. Sether, A. Karasev, W. Borth, J. Hu
- Biology, Medicine
- Archives of Virology
- 19 February 2008
Pineapple mealybug wilt-associated virus-1 (PMWaV-1; family Closteroviridae, genus Ampelovirus) belongs to a complex of mealybug-transmissible viruses found in pineapple worldwide. In this study, the… Expand
Detection of pineapple closterovirus in pineapple plants and mealybugs using monoclonal antibodies
Monoclonal antibodies (MAbs) were produced to the pineapple closterovirus (PCV) in Hawaii. These antibodies were shown to be specific for PCV by decoration of the virus particles in immunosorbent… Expand
Genome organization and phylogenetic relationship of Pineapple mealybug wilt associated virus-3 with family Closteroviridae members
The nucleotide sequence of Pineapple mealybug wilt associated virus-3 (PMWaV-3) (Closteroviridae: Ampelovirus), spanning seven open reading frames (ORFs) and the untranslatable region of the 3′ end… Expand
Nucleotide sequence, genome organization and phylogenetic analysis of pineapple mealybug wilt-associated virus-2.
The genome of pineapple mealybug wilt-associated closterovirus-2 (PMWaV-2) was cloned from double-stranded RNA isolated from diseased pineapple and its sequence determined. The 3'-terminal 14861 nt… Expand