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Physiological Characterization of the ARO10-Dependent, Broad-Substrate-Specificity 2-Oxo Acid Decarboxylase Activity of Saccharomyces cerevisiae
ABSTRACT Aerobic, glucose-limited chemostat cultures of Saccharomyces cerevisiae CEN.PK113-7D were grown with different nitrogen sources. Cultures grown with phenylalanine, leucine, or methionine asExpand
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Metabolic engineering of the E. coli L-phenylalanine pathway for the production of D-phenylglycine (D-Phg).
D-phenylglycine (D-Phg) is an important side chain building block for semi-synthetic penicillins and cephalosporins such as ampicillin and cephalexin. To produce d-Phg ultimately from glucose,Expand
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Short-Term Metabolome Dynamics and Carbon, Electron, and ATP Balances in Chemostat-Grown Saccharomyces cerevisiae CEN.PK 113-7D following a Glucose Pulse
ABSTRACT The in vivo kinetics in Saccharomyces cerevisiae CEN.PK 113-7D was evaluated during a 300-second transient period after applying a glucose pulse to an aerobic, carbon-limited chemostatExpand
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13C-Labeled Gluconate Tracing as a Direct and Accurate Method for Determining the Pentose Phosphate Pathway Split Ratio in Penicillium chrysogenum
ABSTRACT In this study we developed a new method for accurately determining the pentose phosphate pathway (PPP) split ratio, an important metabolic parameter in the primary metabolism of a cell. ThisExpand
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Enzyme Assays: High-throughput Screening, Genetic Selection and Fingerprinting
Nowadays, the characterization of enzymes using simple assays is a key technology for finding the optimum enzymes for, among others, catalysis in enzymology and drug discovery. Thus, enzymes haveExpand
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Metabolic flux and metabolic network analysis of Penicillium chrysogenum using 2D [13C, 1H] COSY NMR measurements and cumulative bondomer simulation.
At present two alternative methods are available for analyzing the fluxes in a metabolic network: (1) combining measurements of net conversion rates with a set of metabolite balances including theExpand
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Serial 13C‐Based Flux Analysis of an l‐Phenylalanine‐Producing E. coli Strain Using the Sensor Reactor
With the aid of the recently developed Sensor reactor system, a series of three subsequent 13C labeling experiments was performed mirroring the l‐phenylalanine (l‐Phe) production phase of aExpand
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Glutathione S-transferases in gastric carcinomas and in adjacent normal gastric epithelium: immunohistochemical and biochemical analyses.
Glutathione S-transferases (GSTs) are enzymes involved in the detoxification of xenobiotics and are divided into four subclasses. Alpha, Mu, Pi and Theta. Most human gastrointestinal tumors containExpand
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NMR structure calculation methods for large proteins Application of torsion angle dynamics and distance geometry/simulated annealing to the 269-residue protein serine protease PB92
Molecular dynamics in torsion angle space together with the well established combination of metric matrix distance geometry and simulated annealing in Cartesian space have been applied to theExpand
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Gradient-purged isotope filter experiments for the detection of bound water in proteins
Abstract A combination of gradient-purged isotope-filtered NMR experiments is presented, which allows for the detection of long-lived bound water molecules in proteins. The discrimination of directExpand
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