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The catalytic mechanism and structure of thymidylate synthase.
Thymidylate synthase (TS, EC 184.108.40.206) catalyzes the reductive methylation of dUMP by CH2H4folate to produce dTMP and H2folate. Knowledge of the catalytic mechanism and structure of TS has increased…
Covalent bond formation between a DNA-cytosine methyltransferase and DNA containing 5-azacytosine.
- D. Santi, A. Norment, C. Garrett
- Biology, ChemistryProceedings of the National Academy of Sciences…
- 1 November 1984
It is demonstrated that azaC-DNA forms a covalent complex with Hpa II methylase, a bacterial enzyme that methylates the internal C of C-C-G-G sequences, which suggests that the inhibition is, at least in part, an active-site directed process and permits a proposal for the structure of the covalents complex.
Mechanism of interaction of thymidylate synthetase with 5-fluorodeoxyuridylate.
Kinetic and catalytic mechanism of HhaI methyltransferase.
Total synthesis of long DNA sequences: synthesis of a contiguous 32-kb polyketide synthase gene cluster.
- Sarah J Kodumal, Kedar G. Patel, R. Reid, Hugo Menzella, M. Welch, D. Santi
- BiologyProceedings of the National Academy of Sciences…
- 20 October 2004
This work has developed and implemented a strategy for the high-throughput synthesis of long, accurate DNA sequences and validated the approach by building a synthetic 31,656-bp polyketide synthase gene cluster whose functionality was demonstrated by its ability to produce the megaenzyme and itspolyketide product in Escherichia coli.
Molecular recognition of tRNA by tRNA pseudouridine 55 synthase.
It is concluded that recognition of tRNA by pseudouridine 55 synthase resides in the conformation of the T-arm plus four specific bases of the loop.
A conserved aspartate of tRNA pseudouridine synthase is essential for activity and a probable nucleophilic catalyst.
It is concluded that the conserved Asp60 is essential for pseudouridine synthase activity and proposed mechanisms which involve this residue in important catalytic roles are proposed.
On the mechanism of inhibition of DNA-cytosine methyltransferases by cytosine analogs
Stereochemical modeling of disulfide bridges. Criteria for introduction into proteins by site-directed mutagenesis.
- R. Sowdhamini, N. Srinivasan, B. Shoichet, D. Santi, C. Ramakrishnan, P. Balaram
- Chemistry, BiologyProtein Engineering
- 1 November 1989
A computer modeling procedure for assessing the stereochemical suitability of pairs of residues in proteins as potential sites for introduction of cystine disulfide crosslinks has been developed and two positions for the introduction of 'stereochemically optimal' disulfides are identified in subtilisin.
Combinatorial polyketide biosynthesis by de novo design and rearrangement of modular polyketide synthase genes
A generic approach to the design of synthetic PKS genes where facile cassette assembly and interchange of modules and domains are facilitated by a repeated set of flanking restriction sites, providing a truly combinatorial approach for the production of polyketides.