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Proteomics of the Chloroplast Envelope Membranes from Arabidopsis thaliana*S
The present work helps understanding chloroplast envelope metabolism at the molecular level and provides a new overview of the biochemical machinery of the chloroplasts envelope membranes.
AT_CHLORO, a Comprehensive Chloroplast Proteome Database with Subplastidial Localization and Curated Information on Envelope Proteins*
A unique knowledge base containing extensive information on the proteins identified in envelope fractions was obtained, allowing new insights into this membrane system to be revealed, and the data obtained provide unexpected information about plastidial or subplastidials localization of some proteins that were not suspected to be associated to this membranes system.
Integral membrane proteins of the chloroplast envelope: Identification and subcellular localization of new transporters
A subcellular proteomic approach to discover new envelope transporters was developed, which combined the use of highly purified and characterized membrane fractions, extraction of the hydrophobic proteins with organic solvents, SDS/PAGE separation, and tandem mass spectrometry analysis.
Chloroplast proteomics and the compartmentation of plastidial isoprenoid biosynthetic pathways.
About 100 enzymes involved in the synthesis of chloroplast-specific isoprenoids, including tetrapyrroles and chlorophyll biosynthesis and breakdown within chloroplasts are focused on.
Non-canonical Transit Peptide for Import into the Chloroplast* 210
It is suggested that a domain of the ceQORH bacterial ancestor may have evolved so as to exclude the general requirement of an N-terminal plastid transit sequence, and its subcellular localization is limited to the inner membrane of the chloroplast envelope.
Persistence across Pleistocene ice ages in Mediterranean and extra-Mediterranean refugia: phylogeographic insights from the common wall lizard
The phylogeographic pattern observed, with 23 reciprocally monophyletic allo- parapatric lineages having a Pleistocene divergence, suggests a scenario of long-term isolation in multiple ice-age refugia across the species distribution range, and provides a paradigm of temperate species survival in Mediterranean and extra-Mediterranean glacialRefugia.
Heterologous Expression of Membrane Proteins: Choosing the Appropriate Host
General recommendations are formulated to assist with choosing an appropriate system for heterologous expression of a membrane protein based on observations of protein behaviour in the different hosts and the results highlight certain factors that should be considered when selecting an expression host.
Large scale investigation of chemical composition, structure and corrosion mechanism of bronze archeological artefacts from Mediterranean basin
A large number of Cu-based archaeological artefacts from the Mediterranean basin have been selected for investigation of their chemical composition, metallurgical features and corrosion products