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Quantitative Detection of Listeria monocytogenes and Listeria innocua by Real-Time PCR: Assessment of hly, iap, and lin02483 Targets and AmpliFluor Technology
Two real-time PCR assays were developed and assessed for the detection and quantification of the food-borne pathogen Listeria monocytogenes and the closely related nonpathogenic species L. innocua and the performance was similar to that of the TaqMan system. Expand
Virus hazards from food, water and other contaminated environments
M Molecular epidemiology and surveillance of environmental samples are necessary to elucidate the public health hazards associated with exposure to environmental viruses and the human pathogenic viruses that are most relevant in this context are nonenveloped. Expand
Analytical Methods for Virus Detection in Water and Food
A survey of the currently available methods for detection of viruses in food and environmental matrices was conducted, gathering information on protocols for extraction of viruses from various matrices and on the various specific detection techniques for each virus type. Expand
Real-time PCR-based methods for detection of Mycobacterium avium subsp. paratuberculosis in water and milk.
A real-time PCR assay for quantitative detection of Mycobacterium avium paratuberculosis has been developed. It targets and amplifies sequences from the IS900 insertion element which is specific forExpand
Simultaneous detection of Listeria monocytogenes and Salmonella by multiplex PCR in cooked ham
A multiplex PCR (m-PCR) assay with an internal amplification control (IAC) was developed for the simultaneous detection of Salmonella spp. and Listeria monocytogenes through invA and prfA genes,Expand
Rapid Quantitative Detection of Listeria monocytogenes in Meat Products by Real-Time PCR
A quick and simple method based on filtration, Chelex-100-based DNA purification, and real-time PCR for the quantitative detection of L. monocytogenes in meat products is described, with excellent accuracy compared to that of the plate count method. Expand
Occurrence of Human Enteric Viruses in Commercial Mussels at Retail Level in Three European Countries
Interestingly, there appeared to be a significant association between the type of mussel species and the positive result of samples, although a complete overlap between country and species examined required this finding to be confirmed including samples of both species from all possible countries of origin. Expand
Simultaneous quantitative detection of Listeria spp. and Listeria monocytogenes using a duplex real-time PCR-based assay.
Results indicate that this assay based on the amplification of the 23S rDNA gene can accurately quantify any mixture of Listeria species and simultaneously unambiguously quantify L. monocytogenes. Expand
Prevalence and transmission of hepatitis E virus in domestic swine populations in different European countries
It is confirmed that HEV is circulating in pig farms from weaners to fatteners and that the reproductive number mathematical defined as R0 is in the same range for all countries studied. Expand
High hydrostatic pressure as emergent technology for the elimination of foodborne viruses
High hydrostatic pressure is a non-thermal technology that eliminates microorganisms with a milder effect on the quality of the foods than that produced by heat treatment. Consequently it can produceExpand