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Nucleotide sequence and genomic organization of melon necrotic spot virus.
Cloned cDNA copies of the genomic RNA of melon necrotic spot virus (MNSV) have been sequenced and the sizes and locations of predicted viral proteins have been deduced. The genome consists of at… Expand
Infectious transcripts from cloned cucumber necrosis virus cDNA: evidence for a bifunctional subgenomic mRNA.
Highly infectious synthetic transcripts from full-length cucumber necrosis virus (CNV) cDNA clones have been prepared. Infections produced by these transcripts, by CNV RNA, and by mutated transcripts… Expand
The ion coupling and organic substrate specificities of osmoregulatory transporter ProP in Escherichia coli.
- S. V. MacMillan, D. Alexander, +4 authors J. Wood
- Biology, Medicine
- Biochimica et biophysica acta
- 20 August 1999
Transporter ProP of Escherichia coli, a member of the major facilitator superfamily, mediates osmoprotective proline or glycine betaine accumulation by bacteria exposed to high osmolality… Expand
Nucleotide sequence of tomato ringspot virus RNA-2.
The sequence of tomato ringspot virus (TomRSV) RNA-2 has been determined. It is 7273 nucleotides in length excluding the 3' poly(A) tail and contains a single long open reading frame (ORF) of 5646… Expand
Rapid de novo generation of defective interfering RNA by cucumber necrosis virus mutants that do not express the 20-kDa nonstructural protein.
- D. Rochon
- Biology, Medicine
- Proceedings of the National Academy of Sciences…
- 15 December 1991
It is generally believed that serial passage at high multiplicity of infection (moi) is required for the generation of defective interfering (DI) particles. High levels of DI RNAs are found… Expand
Complete nucleotide sequence of the cucumber necrosis virus genome.
The complete nucleotide sequence of the cucumber necrosis virus (CNV) genome has been determined. The genome is 4701 nucleotides in length and contains five long open reading frames (ORF). ORF1… Expand
Characterization of a recombinant murine 18.5-kDa myelin basic protein.
- I. Bates, P. Matharu, +6 authors G. Harauz
- Chemistry, Medicine
- Protein expression and purification
- 1 November 2000
A recombinant hexahistidine-tagged 18.5-kDa isoform of murine myelin basic protein has been characterized biochemically and immunogenically, by mass spectrometry, by circular dichroism under various… Expand
Identification of Specific Cucumber Necrosis Virus Coat Protein Amino Acids Affecting Fungus Transmission and Zoospore Attachment
ABSTRACT Cucumber necrosis virus (CNV) is naturally transmitted in the soil by zoospores of the fungal vector Olpidium bornovanus. Successful transmission requires that virus particles attach to the… Expand
Organization of tomato bushy stunt virus genome: characterization of the coat protein gene and the 3' terminus.
We have synthesized cDNA clones of the genome of the cherry strain of tomato bushy stunt virus (TBSV-cherry) and have used them as hybridization probes to identify and position two 3' coterminal… Expand
Deletion analysis of the promoter for the cucumber necrosis virus 0.9-kb subgenomic RNA.
Sequences comprising the core promoter for the cucumber necrosis virus (CNV) 0.9-kb subgenomic RNA have been determined using deletion analysis and site-directed mutagenesis. The deletion studies… Expand