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Clathrin-Mediated Constitutive Endocytosis of PIN Auxin Efflux Carriers in Arabidopsis
It is demonstrated that clathrin-dependent endocytosis is operational in plants and constitutes the predominant pathway for the internalization of numerous plasma-membrane-resident proteins including PIN auxin efflux carriers. Expand
Identification of Multivesicular Bodies as Prevacuolar Compartments in Nicotiana tabacum BY-2 Cells Online version contains Web-only data.
Uptake studies with the styryl dye FM4-64 strongly indicate that PVCs also lie on the endocytic pathway of BY-2 cells, and unequivocally identified MVBs as PVCs in N. tabacum BY- 2 cells. Expand
Endocytic and Secretory Traffic in Arabidopsis Merge in the Trans-Golgi Network/Early Endosome, an Independent and Highly Dynamic Organelle[W]
Examination of secretory and endocytotic trafficking in Arabidopsis provides evidence that both endocytic and secretory cargo pass through the trans-Golgi network/early endosome (TGN/EE) and demonstrates that cargo in late endosomes/multivesicular bodies is destined for vacuolar degradation. Expand
Reevaluation of the Effects of Brefeldin A on Plant Cells Using Tobacco Bright Yellow 2 Cells Expressing Golgi-Targeted Green Fluorescent Protein and COPI Antisera Online version contains Web-only
Although the initial effects of BFA on plant cells are the same as those described for mammalian cells, the secondary and tertiary effects have drastically different morphological manifestations, indicating that there are fundamental differences in the functional architecture and properties of the plant Golgi apparatus. Expand
Multivesicular Bodies Mature from the Trans-Golgi Network/Early Endosome in Arabidopsis[W]
Evidence is presented that post-TGN transport toward lytic vacuoles occurs independently of clathrin and that MVBs/LEs are derived from the TGN/EE through maturation, indicating that MVB originate from theT GN/EE in a process that requires the action of ESCRT for the formation of intraluminal vesicles and annexins for the final step of releasing MVBs as a transport carrier to the vacuole. Expand
Brefeldin A: Deciphering an Enigmatic Inhibitor of Secretion
The fungal macrocyclic lactone brefeldin A has proved to be of great value as an inhibitor of protein trafficking in the endomembrane system of mammalian cells and as a inhibitor of secretion and vacuolar protein transport in plant cells. Expand
Functional diversification of closely related ARF-GEFs in protein secretion and recycling
The results suggest that large ARF-GEFs of the GBF1 class perform a conserved role in endoplasmic reticulum–Golgi trafficking and secretion, which is done by GNL1 and GNOM in Arabidopsis, whereas GNOM has evolved to perform an additional plant-specific function of recycling from endosomes to the plasma membrane. Expand
Subcellular volumes and metabolite concentrations in barley leaves
Metabolite concentrations in subcellular compartments from mature barley (Hordeum vulgare L. cv. Apex) leaves after 9 h of illumination and 5 h of darkness were determined by nonaqueous fractionationExpand
Rice SCAMP1 Defines Clathrin-Coated, trans-Golgi–Located Tubular-Vesicular Structures as an Early Endosome in Tobacco BY-2 Cells[W][OA]
Drug treatments and confocal immunofluorescence studies demonstrated that the punctate cytosolic organelles labeled by YFP-SCAMP1 or SCAMP1 were distinct from the Golgi apparatus and PVCs. Expand
Plant Retromer, Localized to the Prevacuolar Compartment and Microvesicles in Arabidopsis, May Interact with Vacuolar Sorting Receptors[W]
Preliminary data suggesting that retromer interacts with the cytosolic domain of a VSR were obtained by immunoprecipitation experiments performed on detergent-solubilized microsomes with Vps35 antibodies. Expand