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Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: Implications for blastocyst yield and blastocyst quality
TLDR
The results indicate that the intrinsic quality of the oocyte is the main factor affecting blastocyst yields, while the conditions of embryo culture have a crucial role in determining Blastocyst quality. Expand
Bovine Embryo Culture in the Presence or Absence of Serum: Implications for Blastocyst Development, Cryotolerance, and Messenger RNA Expression1
TLDR
It is shown that conditions of postfertilization culture, in particular, the presence of serum in the medium, can affect the speed of embryo development and the quality of the resulting blastocysts. Expand
Analysis of Differential Messenger RNA Expression Between Bovine Blastocysts Produced in Different Culture Systems: Implications for Blastocyst Quality1
TLDR
The study of 7 differentially expressed mRNAs known to be involved in developmental process in the embryo suggests roles for apoptosis, oxidative stress, gap junctions, and differentiation in the determination of embryo quality. Expand
Sex determines the expression level of one third of the actively expressed genes in bovine blastocysts
TLDR
Comparison of gene-expression patterns between male and female blastocysts by DNA microarray comparison suggested a higher global transcriptional level in females and a more active protein metabolism in males, and a gene homolog to an X-linked gene involved in network interactions during spliceosome assembly was found in the Y-Chr. Expand
Temporal Divergence in the Pattern of Messenger RNA Expression in Bovine Embryos Cultured from the Zygote to Blastocyst Stage In Vitro or In Vivo1
TLDR
The results demonstrate that the relative abundance of the transcripts studied varies throughout the preimplantation period and is strongly influenced by the culture environment, and changes in transcript abundance in blastocyst stage embryos are in many cases a consequence of perturbed transcription earlier in development. Expand
Effect of follicle size on bovine oocyte quality and developmental competence following maturation, fertilization, and culture in vitro
TLDR
It was found that administration of six injections of pFSH beginning 3 days prior to slaughter resulted in a significant increase in the proportion of follicles >6 mm in diameter compared to that in nontreated controls and to animals that received only four injection groups. Expand
Developmental, qualitative, and ultrastructural differences between ovine and bovine embryos produced in vivo or in vitro
TLDR
The quality of ovine blastocysts was significantly higher than their bovine counterparts produced under identical in vitro conditions suggesting inherent species differences between these two groups affecting embryo quality. Expand
Oocyte and embryo quality: effect of origin, culture conditions and gene expression patterns.
TLDR
Evidence for an effect of oocyte origin and/or in vitro maturation conditions on the developmental capacity and gene expression patterns in the oocyte is demonstrated and the well-documented effects of post-fertilization culture environment on embryo gene expression and quality are highlighted. Expand
Progesterone and conceptus elongation in cattle: a direct effect on the embryo or an indirect effect via the endometrium?
TLDR
Data provide clear evidence to support the hypothesis that P(4)-induced changes in the uterine environment are responsible for the advancement in conceptus elongation reported previously in cattle and that, interestingly, the embryo does not need to be present during the period of high P( 4) in order to exhibit advanced elongation. Expand
Elevated Non-Esterified Fatty Acid Concentrations during Bovine Oocyte Maturation Compromise Early Embryo Physiology
TLDR
Exposure of maturing oocytes to elevated NEFA concentrations has a negative impact on fertility not only through a reduction in oocyte developmental capacity but through compromised early embryo quality, viability and metabolism. Expand
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