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High cell density cultivation of Escherichia coli at controlled specific growth rate.
The proposed HCDC suppresses generation of inhibitory by-products and the high yield coefficients indicate the economy of the process. Expand
High-cell-density cultivation of microorganisms
Development in the application of multivariate statistical control, artificial neural networks, fuzzy control and knowledge-based supervision (expert systems) are summarized for high-cell-density cultivation, with emphasis on robust fermentation control. Expand
High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions
Abstract Functional bivalent miniantibodies, directed against the epidermal growth factor receptor, accumulated to more than 3 gl−1 in high-cell-density cultures of Escherichia coli RV308(pHKK) on aExpand
High-cell-density cultivation of Escherichia coli.
  • D. Riesenberg
  • Biology, Medicine
  • Current opinion in biotechnology
  • 1 June 1991
High-cell-density cultivations of Escherichia coli in glucose-mineral-salt media produce more than 100 g dry cells litre-1 in special fed-batch modes with feeding of glucose and ammonia only to allow optimum recombinant protein generation. Expand
High cell density fermentation of recombinant Escherichia coli expressing human interferon alpha 1
A defined medium was developed which, by means of a specific fed-batch mode, allows growth of the recombinant Escherichia coli strain TG1 up to a cell density of 60 g dry weight/l, and expressed interferon alpha 1 more efficiently at a lower specific growth rate than at the maximumspecific growth rate. Expand
Effect of ppGpp on Escherichia coli cyclopropane fatty acid synthesis is mediated through the RpoS sigma factor (sigmaS).
It is reported that the effect of RelA on CFA synthesis is indirect, and in vitro and in vivo experiments show no direct interaction between ppGpp and CFA synthase activity. Expand
Expression and secretion of functional miniantibodies McPC603scFvDhlx in cell-wall-less L-form strains of Proteus mirabilis and Escherichia coli : A comparison of the synthesis capacities of L-form
The results show that the processes of correct folding and assembling of the miniAb molecules are possible without the periplasmic compartment. Expand
Influence of stringent and relaxed response on excretion of recombinant proteins and fatty acid composition in Escherichia coli
Results indicate that a change in fatty acid composition of membrane lipids after starvation of cells may be responsible for the prevention of loss of cellular proteins into the culture medium in stringent controlled cells of Escherichia coli. Expand
A radioimmunoassay for (p)ppGpp and its application to Streptomyces hygroscopicus
The very sensitive radioimmunoassay allowed determination of very low amounts: 1–50 pmoles (p)ppGpp per assay tube and the application of the RIA to S. hygroscopicus revealed a basal level in exponentially growing mycelium of about 2 pmoles. Expand
Dependence of macromolecular composition and morphology of Streptomyces hygroscopicus on specific growth rate.
The hypothesis about the increase of the hyphal regions showing high synthesis activity in S. hygroscopicus mycelia grown in glucose-limited continuous cultures with increasing micron was confirmed autoradiographically with respect to protein synthesis and indicates that the morphology is regulated by different mechanisms depending on the specific growth rate. Expand