The Polycomb complex PRC2 and its mark in life
- R. Margueron, D. Reinberg
- Biology, ChemistryNature
- 20 January 2011
This work has uncovered a role for non-coding RNA in the recruitment of PRC2 to target genes, and expanded the perspectives on its function and regulation.
Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein.
- A. Kuzmichev, K. Nishioka, H. Erdjument-Bromage, P. Tempst, D. Reinberg
- BiologyGenes & Development
- 15 November 2002
The isolation of a multiprotein E(z) complex that contains extra sex combs, suppressor of zeste-12, and the histone binding proteins RbAp46/RbAp48 is reported, which possesses HMT activity with specificity for Lys 9 (K9) and Lys 27 (K27) of histone H3.
A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin.
- G. Schotta, M. Lachner, T. Jenuwein
- Biology, ChemistryGenes & Development
- 1 June 2004
Together, the data indicate a function for H4-K20 trimethylation in gene silencing and further suggest H3-K9 and H4,K20 trimmedethylation as important components of a repressive pathway that can index pericentric heterochromatin.
PCGF homologs, CBX proteins, and RYBP define functionally distinct PRC1 family complexes.
- Zhonghua Gao, Jin Zhang, D. Reinberg
- Biology, ChemistryMolecules and Cells
- 10 February 2012
Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation.
- Y. Zhang, H. Ng, H. Erdjument-Bromage, P. Tempst, A. Bird, D. Reinberg
- Biology, ChemistryGenes & Development
- 1 August 1999
A novel polypeptide highly related to the metastasis-associated protein 1, MTA2, and the methyl-CpG-binding domain-containing protein, MBD3, were found to be subunits of the NuRD complex, which may provide a means of gene silencing by DNA methylation.
Role of the polycomb protein EED in the propagation of repressive histone marks
- R. Margueron, N. Justin, S. Gamblin
- BiologyNature
- 8 October 2009
It is shown that the carboxy-terminal domain of EED specifically binds to histone tails carrying trimethyl-lysine residues associated with repressive chromatin marks, and that this leads to the allosteric activation of the methyltransferase activity of PRC2.
FACT Facilitates Transcription-Dependent Nucleosome Alteration
- R. Belotserkovskaya, Sangtaek Oh, V. Bondarenko, G. Orphanides, V. Studitsky, D. Reinberg
- BiologyScience
- 22 August 2003
The FACT facilitates Pol II–driven transcription by destabilizing nucleosomal structure so that one histone H2A-H2B dimer is removed during enzyme passage, defining the mechanism by which Pol II can transcribe through chromatin without disrupting its epigenetic status.
Demethylation of H3K27 Regulates Polycomb Recruitment and H2A Ubiquitination
- Min Gyu Lee, R. Villa, R. Shiekhattar
- Biology, ChemistryScience
- 19 October 2007
It is shown that human UTX, a member of the Jumonji C family of proteins, is a di- and trimethyl H3K27 demethylase, and a concerted mechanism for transcriptional activation in which cycles of H3k4 methylation by MLL2/3 are linked with the demethylation of H 3K27 through UTX.
Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms.
- R. Margueron, Guohong Li, D. Reinberg
- BiologyMolecules and Cells
- 21 November 2008
Human SirT1 interacts with histone H1 and promotes formation of facultative heterochromatin.
- A. Vaquero, Michael B Scher, Donghoon Lee, H. Erdjument-Bromage, P. Tempst, D. Reinberg
- Biology, ChemistryMolecules and Cells
- 8 October 2004
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