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The Polycomb complex PRC2 and its mark in life
TLDR
This work has uncovered a role for non-coding RNA in the recruitment of PRC2 to target genes, and expanded the perspectives on its function and regulation. Expand
A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin.
TLDR
Together, the data indicate a function for H4-K20 trimethylation in gene silencing and further suggest H3-K9 and H4,K20 trimmedethylation as important components of a repressive pathway that can index pericentric heterochromatin. Expand
Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein.
TLDR
The isolation of a multiprotein E(z) complex that contains extra sex combs, suppressor of zeste-12, and the histone binding proteins RbAp46/RbAp48 is reported, which possesses HMT activity with specificity for Lys 9 (K9) and Lys 27 (K27) of histone H3. Expand
PCGF homologs, CBX proteins, and RYBP define functionally distinct PRC1 family complexes.
TLDR
A comprehensive proteomic and genomic analysis uncovered six major groups of PRC1 complexes, each containing a distinct PCGF subunit, a RING1A/B ubiquitin ligase, and a unique set of associated polypeptides. Expand
Analysis of the NuRD subunits reveals a histone deacetylase core complex and a connection with DNA methylation.
TLDR
A novel polypeptide highly related to the metastasis-associated protein 1, MTA2, and the methyl-CpG-binding domain-containing protein, MBD3, were found to be subunits of the NuRD complex, which may provide a means of gene silencing by DNA methylation. Expand
FACT Facilitates Transcription-Dependent Nucleosome Alteration
TLDR
The FACT facilitates Pol II–driven transcription by destabilizing nucleosomal structure so that one histone H2A-H2B dimer is removed during enzyme passage, defining the mechanism by which Pol II can transcribe through chromatin without disrupting its epigenetic status. Expand
Role of the polycomb protein EED in the propagation of repressive histone marks
TLDR
It is shown that the carboxy-terminal domain of EED specifically binds to histone tails carrying trimethyl-lysine residues associated with repressive chromatin marks, and that this leads to the allosteric activation of the methyltransferase activity of PRC2. Expand
Transcription regulation by histone methylation: interplay between different covalent modifications of the core histone tails.
TLDR
This work aims to demonstrate the efforts towards in-situ applicability of EMMARM, which aims to provide real-time information about the “building blocks” of EMT and its role in cancer progression. Expand
Ezh1 and Ezh2 maintain repressive chromatin through different mechanisms.
TLDR
It is reported that the mammalian homologs Ezh1 and Ezh2 form similar PRC2 complexes but exhibit contrasting repressive roles, and Ez h1 knockdown was ineffectual on global H3K27me2/3 levels, while Ezh 1 directly and robustly represses transcription from chromatinized templates and compacts chromatin in the absence of the methyltransferase cofactor SAM. Expand
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