D. Rein

Publications14
h-index8
Citations161
Highly Influential Citations4
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Influence of single-stranded DNA-binding protein on recA induction in Escherichia coli.
Two ssb mutants of Escherichia coli, which carry a lesion in the single-strand DNA-binding protein (SSB), are sensitive to UV-irradiation. We have investigated the influence of SSB on the "SOS"… Expand
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Variability in the nucleic acid binding site size and the amount of single‐stranded DNA‐binding protein in Escherichia coli
The Escherichia coli single‐stranded DNA binding protein (SSB), essential for DNA replication, recombination and repair, can undergo a thermally induced irreversible conformational change which does… Expand
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The product of the lexC gene of Escherichia coli is single-stranded DNA-binding protein.
Extracts from lexC113 cells could not support phage G4 DNA-dependent replication unless supplemented with single-stranded DNA-binding protein. Purified lexC113 binding protein supported synthesis in… Expand
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Interaction of a folded chromosome-associated protein with single-stranded DNA-binding protein of Escherichia coli, identified by affinity chromatography.
A single-stranded DNA-binding protein (SSB) affinity column was prepared by optimizing the coupling of Escherichia coli single-stranded DNA-binding protein to Affi-Gel 10. The bound SSB retained its… Expand
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Effects of ethidium bromide on growth and morphology of Tetrahymena pyriformis.
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A solid-phase assay for antiactin antibody and actin using protein-A.
Abstract A method has been developed for the detection of an immunocomplex between an agaroselinked antigen and its antibody using 125 I-labeled protein-A. This procedure has been used to follow the… Expand
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Actin and Myosin Synthesis During Differentiation of Neuroblastoma Cells
Abstract: The levels and rates of synthesis of actin and myosin in murine neuroblastoma S20 cells have been examined during neurite formation in the absence of serum. Two forms of actin (β, ‐γ) have… Expand
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Suppression of the Escherichia coli ssb-1 mutation by an allele of groEL.
A series of spontaneous suppressors to the temperature-sensitive phenotype of the single-stranded DNA-binding protein mutation ssb-1 were isolated. A genomic library of EcoRI fragments from one of… Expand
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Structure-function analysis of DNA polymerase-beta using monoclonal antibodies: identification of a putative nucleotide binding domain.
DNA polymerase-beta was purified from Novikoff hepatoma and used as an antigen in an in vitro immunization system to produce monoclonal antibodies. These reagents surprisingly showed cross-reactivity… Expand
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Purification and enzymological characterization of DNA-dependent ATPase IV from the Novikoff hepatoma.
DNA-dependent ATPase IV has been purified to near homogeneity from the Novikoff rat hepatoma. The enzyme is devoid of DNA polymerase, RNA polymerase, exonuclease, endonuclease, phosphomonoesterase,… Expand
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