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The Molecular Cloning of Artemisinic Aldehyde Δ11(13) Reductase and Its Role in Glandular Trichome-dependent Biosynthesis of Artemisinin in Artemisia annua*
In an effort to understand double bond reduction in artemisinin biosynthesis, extracts of A. annua flower buds were investigated and found to contain artemisinic aldehyde Δ11(13) double bond reductase activity and the corresponding gene Dbr2, encoding a member of the enoate reduct enzyme family with similarity to plant 12-oxophytodienoates, was found to be highly expressed in glandular trichomes.
Artemisia annua L. (Asteraceae) trichome‐specific cDNAs reveal CYP71AV1, a cytochrome P450 with a key role in the biosynthesis of the antimalarial sesquiterpene lactone artemisinin
Molecular cloning of an aldehyde dehydrogenase implicated in artemisinin biosynthesis in Artemisia annuaThis paper is one of a selection of papers published in a Special Issue from the National…
In a search for alternative enzymes that could catalyze the oxidation of dihydroartemisinic aldehyde, an expressed sequence tag collection from A. annua was investigated for relevant cDNAs and led to the isolation of a full-length cDNA encoding an alde Hyde dehydrogenase homologue, named Aldh1, which is highly expressed in trichomes.
Dissection of the phytohormonal regulation of trichome formation and biosynthesis of the antimalarial compound artemisinin in Artemisia annua plants.
Analysis of phytohormone-modulated transcriptional regulons provides clues to dissect the concerted regulation of metabolism and development of plant trichomes.
Alteration of Seed Fatty Acid Composition by an Ethyl Methanesulfonate-Induced Mutation in Arabidopsis thaliana Affecting Diacylglycerol Acyltransferase Activity
It is demonstrated that changes to triacylglycerol biosynthesis can result in dramatic changes in fatty acid composition and, in particular, in the accumulation of VLCFAs in seed storage lipids.
HPLC Separation of Glucosinolates from Leaves and Seeds of Arabidopsis thaliana and Their Identification Using Thermospray Liquid Chramatography/Mass Spectrometry
Leaf and seed extracts of Arabidopsis thaliana var. Columbia contain a large number of glucosinolates, representing close to 2 5 % of those known to occur in nature. The glucosinolates, in the form…
The production of artemisinin precursors in tobacco.
- Yansheng Zhang, Goska Nowak, D. Reed, P. Covello
- Medicine, ChemistryPlant Biotechnology Journal
- 1 May 2011
Results and in vivo metabolic experiments suggest that amorphane sesquiterpenoid aldehydes are formed, but conditions in the transgenic tobacco cells favour reduction to alcohols rather than oxidation to acids.
Transcriptome analysis based on next-generation sequencing of non-model plants producing specialized metabolites of biotechnological interest.
High-Level Production of γ-Linolenic Acid in Brassica juncea Using a Δ6 Desaturase from Pythium irregulare
Identification of PiD6, a new cDNA from the oleaginous fungus, Pythium irregulare, encoding a 459-amino acid protein that shares sequence similarity to carboxyl-directed desaturases from various species is reported.
Characterization of the Brassica napus extraplastidial linoleate desaturase by expression in Saccharomyces cerevisiae.
The substrate specificity and regioselectivity of the Brassica napus extraplastidial linoleate desaturase (FAD3) was investigated in vivo in a heterologous expression system and indicates that the enzyme has omega-3, as opposed to Delta-15 or double-bond-related regioseslectivity.