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High-performance liquid chromatography analysis of nanomole levels of glutathione, glutathione disulfide, and related thiols and disulfides.
- D. Reed, J. Babson, P. W. Beatty, A. Brodie, W. W. Ellis, D. W. Potter
- Chemistry, Medicine
- Analytical biochemistry
- 15 July 1980
Abstract A rapid and sensitive high-performance liquid chromatography method for determination of nanomole levels of glutathione, glutathione disulfide, cysteine glutathione-mixed disulfide and 20… Expand
High-performance liquid chromatography of thiols and disulfides: dinitrophenol derivatives.
Publisher Summary High-performance liquid chromatography (HPLC) provides a rapid, sensitive, and reproducible means of separating and quantifying simultaneously a variety of sulfur-containing amino… Expand
Glutathione: toxicological implications.
- D. Reed
- Biology, Medicine
- Annual review of pharmacology and toxicology
Glutathione as reduced glutathione (GSH) and as the disulfide (GSSG) is a unique peptide that continues to interest toxicologists-as reflected in the numerous symposia and reviews ( 1-6). This… Expand
Status of the mitochondrial pool of glutathione in the isolated hepatocyte.
Using the selective membrane-solubilizing properties of digitonin and a rapid centrifugation method to separate cytoplasmic and mitochondrial components, the metabolic state of mitochondrial… Expand
Glutathione depletion and susceptibility.
Glutathione redox cycle-driven recovery of reduced glutathione after oxidation by tertiary-butyl hydroperoxide in preimplantation mouse embryos.
The purpose of this study was to determine whether mouse embryos at the two-cell to blastocyst stage have the capacity to reduce glutathione disulfide (GSSG) and to elucidate the mechanism that… Expand
Inactivation of glutathione reductase by 2-chloroethyl nitrosourea-derived isocyanates.
Abstract The specific inactivation of yeast glutathione reductase (GSSG-reductase) by 2-chloroethyl isocyanate and cyclohexyl isocyanate derived from their respective 2-chloroethyl nitrosoureas has… Expand
Involvement of the cystathionine pathway in the biosynthesis of glutathione by isolated rat hepatocytes.
The ability of l-methionine to support glutathione biosynthesis has been investigated in isolated rat hepatocytes under conditions of normal and depleted glutathione status. The addition of… Expand
Radical-induced inactivation of kidney Na+,K(+)-ATPase: sensitivity to membrane lipid peroxidation and the protective effect of vitamin E.
The Na+,K(+)-ATPase is a membrane-bound, sulfhydryl-containing protein whose activity is critical to maintenance of cell viability. The susceptibility of the enzyme to radical-induced membrane lipid… Expand
Cellular recovery of glyceraldehyde-3-phosphate dehydrogenase activity and thiol status after exposure to hydroperoxides.
The activity of the thiol-dependent enzyme glyceraldehyde-3-phosphate dehydrogenase (GPD), in vertebrate cells, was modulated by a change in the intracellular thiol:disulfide redox status. Human lung… Expand