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Polyamine levels during the development of zygotic and somatic embryos of Pinus radiata
Changes in the cellular content of three polyamines (putrescine, spermidine and spermine) were compared at different stages of development in zygotic and somatic embryos of Pinus radiata D. Don.Expand
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Maturation of somatic embryos of Pinus strobus is promoted by a high concentration of gellan gum
Application of somatic embryogenesis to Pinus strobus clonal propagation and genetic improvement was hampered by the difficulty in achieving synchronous maturation of a large number of somaticExpand
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Flow cytometric determination of genome size in Pinus
Abstract The nuclear 2C DNA content of seven Pinus species and Pseudotsuga menziesii was determined by flow cytometric analysis. Conditions for nuclear isolation were adapted to optimise results forExpand
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Cryopreservation of yellow-poplar and sweetgum embryogenic cultures
Cryopreservation has become anessential tool for operational application offorest tree embryogenic cultures, due to thelong evaluation periods needed for treesregenerated from these cultures.Expand
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A biolistic approach for the transfer and expression of a gusA. reporter gene in embryogenic cultures of Pinus radiata
SummaryThe biolistic® particle delivery system was used for the delivery of DNA into embryogenic tissue culture cells of Pinus radiata D. Don. Several experiments with varying parameters wereExpand
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Root Initiation in Cuttings of Pinus radiata
Effects of exogenous growth regulators are related to the histological events in adventitious root initiation in hypocotyls of Pinus radiata D. Don. Indolebutyric acid (IBA) is required both for theExpand
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Light quality treatments enhance somatic seedling production in three southern pine species.
Embryogenic cultures of loblolly pine (Pinus taeda L.), slash pine (Pinus elliottii Engelm.), longleaf pine (Pinus palustris Mill.) and slash pine x longleaf pine hybrids were initiated from immatureExpand
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Root Initiation in Cuttings of Pinus radiata Seedlings II. GROWTH REGULATOR INTERACTIONS
Effects of exogenous growth regulators are related to the histological events in adventitious root initiation in hypocotyls of Pinus radiata D. Don. Indolebutyric acid (IBA) is required both for theExpand
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