• Publications
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Polymer-based gene delivery with low cytotoxicity by a unique balance of side-chain termini.
Protein expression after delivery of plasmid DNA to the cell nucleus depends on the processes of transcription and translation. Cytotoxic gene-delivery systems may compromise these processes andExpand
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Engineered bacterial outer membrane vesicles with enhanced functionality.
We have engineered bacterial outer membrane vesicles (OMVs) with dramatically enhanced functionality by fusing several heterologous proteins to the vesicle-associated toxin ClyA of Escherichia coli.Expand
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Poly(lactic acid)-poly(ethylene glycol) nanoparticles as new carriers for the delivery of plasmid DNA.
The purpose of the present work was to produce and characterize poly(lactic acid)-poly(ethylene glycol) (PLA-PEG) nanoparticles (size lower than 300 nm) containing a high loading of plasmid DNA in aExpand
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Polymers for gene delivery across length scales
  • D. Putnam
  • Materials Science, Medicine
  • Nature materials
  • 1 June 2006
A number of human diseases stem from defective genes. One approach to treating such diseases is to replace, or override, the defective genes with normal genes, an approach called 'gene therapy'.Expand
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PLGA microspheres containing plasmid DNA: preservation of supercoiled DNA via cryopreparation and carbohydrate stabilization.
Biodegradable microspheres containing plasmid DNA have potential uses as mediators of transfection in cells, particularly phagocytic cells such as macrophages. However, the hydrophilic nature and theExpand
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Biomaterial microarrays: rapid, microscale screening of polymer-cell interaction.
The identification of biomaterials that induce optimal gene expression patterns and allow for appropriate levels of cellular attachment is of central importance in tissue engineering and cellExpand
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Polyhistidine-PEG:DNA nanocomposites for gene delivery.
Complexation of plasmid DNA with polycations is a popular method by which to transfer therapeutic nucleic acid sequences to cells. One caveat of the approach is that the positive zeta potential ofExpand
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Cell affinity separations using magnetically stabilized fluidized beds: erythrocyte subpopulation fractionation utilizing a lectin-magnetite support.
A magnetically stabilized fluidized bed is used to separate erythrocyte subpopulations. Binding specificity was obtained by immobilizing the lectin Helix pomatia Agglutinin (HpA) or GriffoniaExpand
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Design of imidazole-containing endosomolytic biopolymers for gene delivery.
The development of safe and effective gene delivery agents poses a great challenge in the quest to make human gene therapy a reality. Cationic polymers represent one important class of materials forExpand
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