D. Prasher

Publications37
h-index18
Citations13,696
Highly Influential Citations548
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Green fluorescent protein as a marker for gene expression.
TLDR
A complementary DNA for the Aequorea victoria green fluorescent protein produces a fluorescent product when expressed in prokaryotic or eukaryotic cells, which can be used to monitor gene expression and protein localization in living organisms.
Removal of a cryptic intron and subcellular localization of green fluorescent protein are required to mark transgenic Arabidopsis plants brightly.
The green fluorescent protein (GFP) from the jellyfish Aequorea victoria is finding wide use as a genetic marker that can be directly visualized in the living cells of many heterologous organisms. We…
Wavelength mutations and posttranslational autoxidation of green fluorescent protein.
TLDR
The availability of two visibly distinct colors should significantly extend the usefulness of GFP in molecular and cell biology by enabling in vivo visualization of differential gene expression and protein localization and measurement of protein association by fluorescence resonance energy transfer.
Primary structure of the Aequorea victoria green-fluorescent protein.
Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein.
TLDR
The characterization of the Aequorea victoria GFP chromophore is described, which is released as a hexapeptide upon digestion of the protein with papain, formed upon cyclization of the residues Ser-dehydroTyr-Gly within the polypeptide.
Using GFP to see the light.
  • D. Prasher
  • Biology, Chemistry
    Trends in genetics : TIG
  • 1 August 1995
Microsatellite DNA Variation among Asian and North American Gypsy Moths (Lepidoptera: Lymantriidae)
TLDR
Variation in the North American population was lower than in Asia, presumably because of the recent population bottleneck associated with the release of European moths in North America.
Physical and biochemical characterization of cloned sbcB and xonA mutations from Escherichia coli K-12
TLDR
The results suggest that the sbcB/xonA gene product is a bi- or multifunctional protein that interacts with single-stranded DNA and possibly with other proteins in the suppression of genetic recombination and DNA-repair deficiencies in recB and recC mutants.
Application of a DNA barcode using the 16S rRNA gene to diagnose pest Arion species in the USA
Arion Ferussac, 1819, is a large, predominantly western Palearctic genus of slugs with a number of species that are of considerable agricultural and forestry concern. Many Arion species have been…
Cloning and expression of the cDNA coding for aequorin, a bioluminescent calcium-binding protein.
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