D. Prüfer

Publications16
h-index6
Citations174
Highly Influential Citations12
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Synthesis of a full-length infectious cDNA clone of cucurbit aphid-borne yellows virus and its use in gene exchange experiments with structural proteins from other luteoviruses.
A full-length cDNA of cucurbit aphid-borne yellows virus (CABYV) has been constructed and expressed either as an in vitro transcript, under control of a bacteriophage T7 RNA polymerase promoter, or… Expand
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Proteomic analysis of latex from the rubber‐producing plant Taraxacum brevicorniculatum
Many plants produce latex, a specialized, metabolically active cytoplasm. This is generally regarded as a defensive trait but latex may also possess additional functions. We investigated the role of… Expand
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In vivo expression of a full-length cDNA copy of potato leafroll virus (PLRV) in protoplasts and transgenic plants
Abstract A full-length cDNA copy (PLRVfl) of potato leafroll virus (PLRV) was constructed and examined in vivo for its biological activities by transient expression experiments with plasmid DNA or in… Expand
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In planta agroinfection by Canadian and German Potato leafroll virus full-length cDNAs1
Full-length infectious cDNA clones of Canadian (PLRVflc) and German (PLRVflg) Potato leafroll virus isolates were constructed and their biological activities examined in planta. For both constructs,… Expand
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MOLECULAR STRATEGIES FOR ENGINEERING RESISTANCE TO POTATO VIRUSES
An increasing number of molecular strategies are available for engineering virus resistance in potatoes (Solatium tuberosum ssp. tuberosum L.) using pathogen-derived or other virus-inhibiting nucleic… Expand
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A novel C-S lyase from the latex-producing plant Taraxacum brevicorniculatum displays alanine aminotransferase and l-cystine lyase activity.
We isolated a novel pyridoxal-5-phosphate-dependent l-cystine lyase from the dandelion Taraxacum brevicorniculatum. Real time qPCR analysis showed that C-S lyase from Taraxacum brevicorniculatum… Expand
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Evolution and Management of the Irish Potato Famine Pathogen Phytophthora Infestans in Canada and the United States
Late blight, caused by Phytophthora infestans (Mont.) de Bary, is the most historically significant and economically destructive disease of potatoes (Solanum tuberosum L.). In addition to potato, P.… Expand
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African descents are more sensitive than European descents to the antitumor compounds α-hederin and kalopanaxsaponin I.
α-Hederin, a natural triterpene saponin and its derivative kalopanaxsaponin I (ksI) exhibit cytotoxicity against various cancer cell lines and IN VIVO tumors. We studied the genetic variants… Expand
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Polerovirus ORF0 genes induce a host-specific response resembling viral infection
Constitutive expression of the ORF0 (open reading frame 0) gene product from the Potato leafroll virus (PLRV) in transgenic host potato plants is known to produce viral-disease-like symptoms. To… Expand
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The Major Floral Promoter NtFT5 in Tobacco (Nicotiana tabacum) Is a Promising Target for Crop Improvement
The FLOWERING LOCUS T (FT)-like gene family encodes key regulators of flower induction that affect the timing of reproduction in many angiosperm species. Agricultural research has therefore focused… Expand
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