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Cost-effective production of a vaginal protein microbicide to prevent HIV transmission
TLDR
The data show that the HIV-neutralization capability of the antibody is equal to or superior to that of the same antibody produced in CHO cells, and it is concluded that this protein production system may provide a means to achieve microbicide ingredient manufacture at costs that would allow product introduction and manufacture in the developing world.
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New downstream processing strategy for the purification of monoclonal antibodies from transgenic tobacco plants.
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Structure‐guided alteration of coenzyme specificity of formate dehydrogenase by saturation mutagenesis to enable efficient utilization of NADP+
TLDR
The results demonstrate that the combined polar interactions and steric factors comprise the main structural determinants responsible for coenzyme specificity.
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Asymptomatic rectal colonization with carbapenem-resistant Enterobacteriaceae and Clostridium difficile among residents of a long-term care facility in New York City.
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[Neonatal screening in Europe revisited: An ISNS-perspective on the current state and developments since 2010].
TLDR
Between 2010 and 2020, most NBS programmes in geographical Europe have matured considerably, both in terms of methodology (modernised) and with regards to the panel of conditions screened (expanded).
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Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from tobacco extract.
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High yield expression, refolding, and characterization of recombinant interferon alpha2/alpha8 hybrids in Escherichia coli.
TLDR
This work describes a protein expression system for the production of IFNs alpha2, alpha8, and their hybrids in insoluble form in Escherichia coli, coupled to an efficient two-step optimized refolding and histidine-tag purification protocol.
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Mechanisms of action of interferon and nucleoside analogues.
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Application of a PEG/salt aqueous two‐phase partition system for the recovery of monoclonal antibodies from unclarified transgenic tobacco extract
TLDR
ATPS was shown to be suitable for initial protein recovery and partial purification of mAb from unclarified transgenic tobacco crude extract and was successfully integrated in an affinity‐based purification protocol, yielding antibodies of high purity and yield.
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Engineering sensitive glutathione transferase for the detection of xenobiotics.
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