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Complete sequence of a picorna-like virus of the genus Iflavirus replicating in the mite Varroa destructor.
To determine whether VDV-1 replicates in mites, a selective RT-PCR was done to detect the presence of the negative-sense RNA strand and the virus isolate could be discriminated by two primer sets, each specific to one virus. Expand
Distinct levels of specificity in thrips transmission of tospoviruses.
Frankliniella occidentalis appeared to be the most efficient vector for the four tospovirus species tested, and Enzyme-linked immunosorbent assay (ELISA) showed that virus could be readily detected in transmitting adult thrips. Expand
The S RNA segment of tomato spotted wilt virus has an ambisense character.
The complete nucleotide sequence of the S RNA of tomato spotted wilt virus (TSWV) was determined and the absence of significant sequence homology between T SWV and bunyaviruses infecting animals suggests that TSWV should be considered as a representative of a new genus within the Bunyaviralidae. Expand
Molecular and serological characterization of iris yellow spot virus, a new and distinct tospovirus species.
Both serological comparisons and sequence determination of the S RNA demonstrate that this virus represents a new and distinct species, belonging to a separate serogroup, and for which the name iris yellow spot virus (IYSV) is proposed. Expand
The nucleotide sequence of the M RNA segment of tomato spotted wilt virus, a bunyavirus with two ambisense RNA segments.
Significant sequence homology was found between the G1 glycoproteins of members of the genus Bunyavirus and a corresponding region in the glycoprotein precursor of TSWV, indicating a close evolutionary relationship between these viruses. Expand
Herbivore arthropods benefit from vectoring plant-viruses
It is proposed that plant pathogens in general have evolved mechanisms to overcome plant defences against their vectors, thus promoting pathogen spread. Expand
Characterization of a Tospovirus Isolate of Iris Yellow Spot Virus Associated with a Disease in Onion Fields in Brazil.
A tospovirus from onion causing a disease known as "sapeca" by growers in Brazil was characterized and it was demonstrated that this virus was serologically related to iris yellow spot virus (IYSV), a toSpovirus recently described in the Netherlands. Expand
Multiplication of tomato spotted wilt virus in its insect vector, Frankliniella occidentalis.
The accumulation of two proteins, the nucleocapsid (N) protein and a non-structural (NSs) protein both encoded by the S RNA of tomato spotted wilt virus (TSWV), was followed in larvae duringExpand
Classification of tospoviruses based on phylogeny of nucleoprotein gene sequences.
The results obtained support the conclusion that, in addition to the species TSWV and INSV, the serogroup II isolates BR-03 and SA-05 have to be considered as distinct species within the genus Tospovirus for which the names tomato chlorotic spot virus and groundnut ringspot virus, respectively, are proposed. Expand
The nonstructural NSm protein of tomato spotted wilt virus induces tubular structures in plant and insect cells.
The efficient formation of NSm-containing tubules emerging from the surface of both cell types indicate that no plant-specific cell structures or proteins are involved in their development. Expand