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Identification of Heterodera glycines using PCR with sequence characterised amplified region (SCAR) primers
A single randomly amplified polymorphic DNA marker species-specific to the soybean cyst nematode species, Heterodera glycines, was identified and developed sets of SCAR primers were successfully used in straightforward, fast and reliable PCR assays to identify H. glycines. Expand
A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR
A method for rapid identification of juveniles and cysts of the soybean cyst nematode based on PCR with species specific primers is described and a single cyst or second stage juvenile of Heterodera glycines alone or in a mixture with other soil inhabiting nematodes was detectable. Expand
First Report of the Cyst Nematode (Heterodera filipjevi) on Wheat in Henan Province, China.
Cyst nematodes obtained from soil samples and plant samples at these four locations differed from those of H. avenae and had uniform morphological and molecular characteristics, and indicated that the four populations were H. filipjevi, a member of the 'Heterodera avenae Group' of cereal cyst Nematodes. Expand
A new pathotype characterization of Daxing and Huangyuan populations of cereal cyst nematode (Heterodera avenae) in China
Because the pathotypes of the two tested CCN populations in Beijing and Qinghai were not identical to any of the 13 pathotypes previously characterized by the test assortment, Daxing and Huangyuan populations were classified as the new pathotypes, named Ha91. Expand
Occurrence and Development of the Cereal Cyst Nematode (Heterodera avenae) in Shandong, China.
Investigation of the population dynamics of H. avenae in the winter wheat cultivars in Shandong provides important information indicating that J2 populations in roots and soil increased after the wheat winter dormancy period. Expand
First Report of Soybean Cyst Nematode (Heterodera glycines) on Soybean from Gansu and Ningxia China
Novel Pectate Lyase Genes of Heterodera glycines Play Key Roles in the Early Stage of Parasitism
Four novel cDNAs that encode pectate lyases were cloned and characterized from the soybean cyst nematode, Heterodera glycines, and the finding that the expression of these genes was strong in the egg, pre-parasitic second-stage juvenile (J2) and early parasitic J2 stages and that it declined in further developmental stages of the nematodes suggests that these proteins play a role in the migratory phase of the Nemesis life cycle. Expand
First Report of the Cyst Nematode (Heterodera elachista) on Rice in Hunan Province, China.
During a survey for the cyst nematodes (Heterodera elachista) from May to June of 2011, cysts detected in hilly rice fields in five counties of Hunan Province, China had uniform morphological and molecular characteristics and indicated that the five populations were H. elachistas belonging to the 'cyperi' group. Expand
Maize growth responses to soil microbes and soil properties after fertilization with different green manures
- J. Tao, X. Liu, +9 authors Huaqun Yin
- Biology, Medicine
- Applied Microbiology and Biotechnology
- 1 February 2017
The results indicate that soil pH, alkali solution nitrogen, and available potassium were the key environmental factors shaping soil bacterial communities and determining maize yields and might be useful as indicators of soil quality and potential crop yield. Expand
Identification and molecular characterization of a new β-1,4-endoglucanase gene (Ha-eng-1a) in the cereal cyst nematode Heterodera avenae
The results indicated that this β-1,4-endoglucanase gene may play a crucial role in plant cell wall-degradation during penetration and migration of nematodes in the host roots. Expand