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Complex Biochemistry and Biotechnological Production of Betalains
This paper synthesizes the results of recent studies on betalain biosynthesis, chemical properties, sources, biotechnology and applications, which suggest optimization of pigment production seems necessary.
Evaluation of Protein Extraction Methods for Proteomic Analysis of Non-Model Recalcitrant Plant Tissues
Although phenol extraction was more time-consuming than the other two methods, it gave almost two-fold higher protein yield, and spectral analysis showed less contamination, leading to the conclusion that the phenol method is the most suitable protein extraction method for these non-model and recalcitrant plant tissues.
Growth Conditions in In Vitro Culture Can Induce Oxidative Stress in Mammillaria gracilis Tissues
- B. Balen, M. Tkalec, D. Pavoković, B. Pevalek-Kozlina, M. Krsnik-Rasol
- BiologyJournal of Plant Growth Regulation
The results showed the appearance of a prominent oxidative stress in HC, HS, and TT as well as a strong induction of the antioxidant system indicating that activated oxygen metabolism could be involved in habituation and hyperhydricity and linked to the loss of tissue organization in M. gracilis.
Protein glycosylation in sugar beet cell line can be influenced by DNA hyper- and hypomethylating agents
It is suggested that hypermethylation and hypomethylation of genomic DNA in sugar beet cells affect protein glycosylation patterns and cellular metabolism, possibly in a mechanism similar to that existing in animal cells.
Identification and quantification of phenolic compounds in Hypericum perforatum L. transgenic shoots
- O. Tusevski, J. Petreska Stanoeva, M. Stefova, D. Pavoković, S. Gadzovska Simic
- ChemistryActa Physiologiae Plantarum
- 6 July 2014
Regeneration of transgenic shoots was achieved from Hypericum perforatum L. hairy roots on hormone-free MS/B5 medium for a period of 4 weeks under a photoperiod of 16-h light. A control experiment…
Plant-mediated asymmetric reduction of 1-(3,4-dimethylphenyl)ethanone
Enhancement of betanin yield in transformed cells of sugar beet (Beta vulgaris L.)
In this system, sucrose affected betanin yield moreÂ strongly than medium modifications or elicitors, and Yeast extract could be used for reverse-Â sequestration ofbetanin where the cells can be used over an extended period.
Morphological and proteomic analyses of sugar beet cultures and identifying putative markers for cell differentiation
- D. Pavoković, D. Poljuha, A. Horvatić, N. Ljubešić, D. Hagège, M. Krsnik-Rasol
- BiologyPlant Cell, Tissue and Organ Culture (PCTOC)
Electron microscopy showed that the ultrastructure of N cells corresponds to that of parenchyma cells, and that these cells contain plastids with large starch grains, and differential expression of these proteins suggests that these play a role in cell line-specific cell wall composition and cell-to-cell adhesion.
Comparison between enhanced MALDI in-source decay by ammonium persulfate and N- or C-terminal derivatization methods for detailed peptide structure determination.
Multiply stage MS after APS addition showed enhanced sensitivity, resolution, and mass accuracy compared to peptide derivatization (SPITC and Lys-tag) or conventional MS and MS(2) analyses and offered more detailed insight into peptide structure.
Light-dependent betanin production by transformed cells of sugar beet.
The tumour line seems to be promising as an alternative source of betanin as well as a model to study sugar-regulated genes involved in tissue morphology control and to study metabolic changes associated with the transformation.