Cucumber mosaic virus-encoded 2b suppressor inhibits Arabidopsis Argonaute1 cleavage activity to counter plant defense.
It is concluded that 2b blocks AGO1 cleavage activity to inhibit miRNA pathways, attenuate RNA silencing, and counter host defense, providing insight on the molecular arms race between host antiviral RNAsilencing and virus counterdefense.
Non-CG methylation patterns shape the epigenetic landscape in Arabidopsis.
It is shown that a poorly characterized methyltransferase, CMT2, is a functional methyl transferase in vitro and in vivo and suggest that non-CG methylation patterns are critical in shaping the landscapes of histone modification and small noncoding RNA.
A selective jumonji H3K27 demethylase inhibitor modulates the proinflammatory macrophage response
It is demonstrated that the first small-molecule catalytic site inhibitor that is selective for the H3K27me3-specific JMJ subfamily binds in a novel manner and reduces lipopolysaccharide-induced proinflammatory cytokine production by human primary macrophages, a process that depends on both JMJD3 and UTX.
How chromatin-binding modules interpret histone modifications: lessons from professional pocket pickers
- Sean D. Taverna, Haitao Li, A. Ruthenburg, C. Allis, D. Patel
- BiologyNature Structural &Molecular Biology
- 1 November 2007
Key features in molecular recognition of histone PTMs by a diverse family of 'reader pockets', highlighting specific readout mechanisms for individual marks, common themes and insights into the downstream functional consequences of the interactions are summarized.
Molecular basis for site-specific read-out of histone H3K4me3 by the BPTF PHD finger of NURF
The molecular basis for specific recognition of H3(1–15)K4me3 by a plant homeodomains (PHD) finger of human BPTF (bromodomain and PHD domain transcription factor) is demonstrated and key specificity-determining residues from binding studies are identified.
Structural basis for discriminative regulation of gene expression by adenine- and guanine-sensing mRNAs.
Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex
- Yanli Wang, S. Juranek, Haitao Li, G. Sheng, T. Tuschl, D. Patel
- Chemistry, BiologyNature
- 18 December 2008
Consistent with the geometry of the ternary complex, bulges residing in the seed segments of the target, but not the guide strand, were better accommodated and their complexes were catalytically active.
Multivalent engagement of chromatin modifications by linked binding modules
- A. Ruthenburg, Haitao Li, D. Patel, C. Allis
- BiologyNature reviews. Molecular cell biology
- 1 December 2007
It is proposed that multivalent interactions on a single histone tail and beyond may have a significant, if not dominant, role in chromatin transactions.
Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes
The slicer activity of the RNA-induced silencing complex resides within its Argonaute (Ago) component, in which the PIWI domain provides the catalytic residues governing guide-strand mediated…