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A Cyanobacterial Chlorophyll Synthase-HliD Complex Associates with the Ycf39 Protein and the YidC/Alb3 Insertase[W][OPEN]
TLDR
A link between chlorophyll biosynthesis and the Sec/YidC-dependent cotranslational insertion of nascent photosystem polypeptides into membranes is revealed and it is expected that this close physical linkage coordinates the arrival of pigments and nascent apoproteins to produce photosynthetic pigment-protein complexes with minimal risk of accumulating phototoxic unbound chlorophyLLs. Expand
Conserved Chloroplast Open-reading Frame ycf54 Is Required for Activity of the Magnesium Protoporphyrin Monomethylester Oxidative Cyclase in Synechocystis PCC 6803
TLDR
Ycf54 is essential for the activity and stability of the oxidative cyclase, and levels of the cyclase component Sll1214 and the Chl biosynthesis enzymes Mg-protoporphyrin IX methyltransferase and protochlorophyllide reductase are significantly impaired in the ycf54 mutant. Expand
Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of photosystem II
TLDR
Cyanobacteria that are able to use FRL for photosynthesis have evolved a novel far-red light photoacclimation (FaRLiP) mechanism to gain a strong selective advantage over other cyanobacteria. Expand
Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities
TLDR
The SCRR–SIP technique may provide a direct method for screening environmental samples, and could help to reveal the ecophysiology of hitherto-unculturable microorganisms, linking microbial species to their ecological function in the natural environment. Expand
Identification of an 8-vinyl reductase involved in bacteriochlorophyll biosynthesis in Rhodobacter sphaeroides and evidence for the existence of a third distinct class of the enzyme.
TLDR
The results of the present study demonstrate that Rsp_3070 is a functional C8-vinyl reductase and that R. sphaeroides utilises at least two enzymes to catalyse this reaction, indicating the existence of a third class, while there remains no direct evidence for the activity of BchJ. Expand
Complete enzyme set for chlorophyll biosynthesis in Escherichia coli
TLDR
A minimum set of enzymes required to make chlorophyll is delineated and a platform for engineering photosynthesis in a heterotrophic model organism is established and established. Expand
Elucidation of the preferred routes of C8-vinyl reduction in chlorophyll and bacteriochlorophyll biosynthesis
TLDR
In vivo data indicate that the preferred substrates for both classes of the enzyme is C8-vinyl chlorophyllide, with C8 -vinyl protochlorophyllid reduced only under conditions in which this pigment accumulates as a result of perturbed formation of chlorophyLLide. Expand
Absence of the cbb3 Terminal Oxidase Reveals an Active Oxygen-Dependent Cyclase Involved in Bacteriochlorophyll Biosynthesis in Rhodobacter sphaeroides
TLDR
It is proposed that the O2-dependent form allows for the biosynthesis of a low level of bacteriochlorophyll under oxic conditions, so that a rapid initiation of photosynthetic processes is possible for this bacterium upon a reduction of oxygen tension. Expand
Progress and challenges in engineering cyanobacteria as chassis for light‐driven biotechnology
TLDR
This commentary article discusses the great progress that has been made in engineering cyanobacterial hosts as microbial cell factories for solar‐powered biosynthesis, and focuses on some of the main areas where the synthetic biology and metabolic engineering tools in cyanobacteria are not as advanced as those in more widely used heterotrophic chassis. Expand
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