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Functional characterization of GcpE, an essential enzyme of the non‐mevalonate pathway of isoprenoid biosynthesis
TLDR
Recombinant GcpE protein from the hyperthermophilic bacterium Thermus thermophilus was produced in Escherichia coli and purified under dioxygen‐free conditions and was enzymatically active in converting 2‐C‐methyl‐D‐erythritol‐2,4‐cyclodiphosphate into HMBPP in the presence of dithionite as reductant. Expand
Replacing the pyrophosphate group of HMB-PP by a diphosphonate function abrogates Its potential to activate human γδ T cells but does not lead to competitive antagonism
The immunological characterization of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMB-PP), and its methylenediphosphonate analogue, HMB-PCP, is described. With an EC(50) of 0.1-0.2 nM, HMB-PP isExpand
Influence of oxidative and nitrosative stress on accumulation of diphosphate intermediates of the non-mevalonate pathway of isoprenoid biosynthesis in corynebacteria and mycobacteria
TLDR
A possible role of the MEP-pathway of isoprenoid biosynthesis and a role of its central intermediate MEcDP in bacterial response to nitrosative and oxidative stress is discussed. Expand
Properties of 2-C-Methyl-D-Erythritol 2,4-Cyclopyrophosphate, an Intermediate in Nonmevalonate Isoprenoid Biosynthesis
TLDR
It is suggested that MEC acts as an endogenous stabilizing agent for bacterial cells subjected to oxidative stress and as an immunomodulator for eukaryotic hosts. Expand
F1-like ATPase from anaerobic bacterium Lactobacillus casei contains six similar subunits.
TLDR
It is concluded that the detachable part of membrane N,N×-dicyclohexylcarbodiimide-sensitive ATPase of L. casei is composed of six similar subunits, which may be regarded as an evolutionary precursor of the catalytic part of the H+ -ATP synthase of aerobic and photosynthetic bacteria, mitochondria, and chloroplasts. Expand
Infrared spectra of outer and cytoplasmic membranes of Escherichia coli
Abstract Outer and cytoplasmic membranes of Escherichia coli were prepared by a method based on isopyenic centrifugation on a sucrose gradient. The infrared spectra of solid films of these membranesExpand
Some peculiarities of functioning of H+-ATPase from the membranes of the anaerobic bacterium Lactobacillus casei.
TLDR
Radiation inactivation analysis gave the target sizes of 176 +/- 5 kDa and 275 +/- 33 kDa for ATPase from anaerobic Lactobacillus casei and aerobic Micrococcus luteus bacteria respectively suggesting essential differences in the operating mechanism for L. casei ATPase and F1 ATPase. Expand
Replacing the pyrophosphate group of HMB-PP by a diphosphonate function abrogates Its potential to activate human gammadelta T cells but does not lead to competitive antagonism.
TLDR
The immunological characterization of (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate, and its methylenediphosphonate analogue, HMB-PCP, is described, which is significantly more potent in stimulating human Vgamma9/Vdelta2 T cells than any other compound described so far. Expand
The main pigment of the dormant Mycobacterium smegmatis is porphyrin.
TLDR
Based on 1H-NMR, MALDI and UV spectra, the fluorescent compounds, extracted from the culture medium as well as from the dormant cells, were concluded to be a mixture of free coproporphyrin III and uroporphyrs III and their corresponding methyl esters. Expand
Subunit composition of the H+-ATPase complex from anaerobic bacterium Lactobacillus casei.
TLDR
The crossed-immunoelectrophoresis of the 14C-labelled ATPase complex against antibodies to a highly purified soluble ATPase has been used and the subunit composition of the complex has been established by autoradiography. Expand
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