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The genome of the square archaeon Haloquadratum walsbyi : life at the limits of water activity
TLDR
Although no direct genetic indication was found that can explain how this peculiar organism retains its square shape, the genome revealed several unique adaptive traits that allow this organism to thrive in its specific and extreme niche. Expand
Unfolding pathways of individual bacteriorhodopsins.
TLDR
Atomic force microscopy and single-molecule force spectroscopy were combined to image and manipulate purple membrane patches from Halobacterium salinarum, revealing the individuality of the unfolding pathways. Expand
Lipid patches in membrane protein oligomers: crystal structure of the bacteriorhodopsin-lipid complex.
TLDR
Analysis of single crystals by nano-electrospray ionization-mass spectrometry demonstrated a preservation of the purple membrane lipid composition in these BR crystals. Expand
Rhodopsin-like protein from the purple membrane of Halobacterium halobium.
TLDR
It is shown that the purple colour is due to retinal bound to an opsin-like protein, the only protein present in this membrane fragment, which has been isolated in relatively pure form from Halobacterium halobium. Expand
Functions of a new photoreceptor membrane.
TLDR
Preliminary results indicate that the gradient in H. halobium plays the central role in energy coupling attributed to such electrochemical gradients by Mitchell's chemiosmotic theory. Expand
Structure of the light-driven chloride pump halorhodopsin at 1.8 A resolution.
TLDR
A combination of ion-ion and ion-dipole interactions for stabilizing the anion 18 angstroms below the membrane surface explains why chloride and proton translocation modes are mechanistically equivalent in archaeal rhodopsins. Expand
Lag1p and Lac1p are essential for the Acyl-CoA-dependent ceramide synthase reaction in Saccharomyces cerevisae.
TLDR
LAG1 and LAC1 are the first genes to be identified that are required for the fumonisin B1-sensitive and acyl-CoA-dependent ceramide synthase reaction, suggesting that aureobasidin A may be toxic because it leads to increased ceramide levels. Expand
Absolute SILAC for accurate quantitation of proteins in complex mixtures down to the attomole level.
TLDR
A new variant of the stable isotope labeling by amino acids in cell culture (SILAC) technique termed "Absolute SILAC" is presented that allows accurate quantitation of selected proteins in complex mixtures and shows that it is possible to quantify over several orders of magnitude, even in the background of a whole cell lysate. Expand
Evolution in the laboratory: the genome of Halobacterium salinarum strain R1 compared to that of strain NRC-1.
TLDR
The sequence of the Halobacterium salinarum strain R1 chromosome and its four megaplasmids is reported and it is concluded that strains R1 and NRC-1 originate from the same natural isolate. Expand
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