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CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations.
T tandem pairs of CBS domains from AMP-activated protein kinase, IMP dehydrogenase-2, the chloride channel CLC2, and cystathionine beta-synthase bind AMP, ATP, or S-adenosyl methionine, while mutations that cause hereditary diseases impair this binding. Expand
3-Phosphoinositide-dependent protein kinase-1 (PDK1): structural and functional homology with the Drosophila DSTPK61 kinase
Human PDK1 is homologous to the Drosophila protein kinase DSTPK61, which has been implicated in the regulation of sex differentiation, oogenesis and spermatogenesis and is likely to mediate the activation of PKB by insulin or growth factors. Expand
The high-resolution crystal structure of a parallel-stranded guanine tetraplex.
The structure of a parallel-stranded tetraplex formed by the hexanucleotide d(TG4T) and stabilized by sodium cations was determined by x-ray crystallography to 1.2 angstroms resolution. Expand
Solution structure of a DNA-binding domain from HMG1.
Inspection of the fold shows that the majority of conserved residue positions in the HMG box family are those involved in maintaining the tertiary structure and thus all homologous HMG boxes probably have essentially the same fold. Expand
Quantitative analysis of chromatin compaction in living cells using FLIM–FRET
FRET analysis of cell lines expressing fluorescently tagged histones on separate nucleosomes demonstrates that variations in chromosome compaction occur during mitosis.
Solution structure of a pair of complement modules by nuclear magnetic resonance.
A portion of human complement factor H spanning the 15th (H15) and 16th (H16) of its 20 modules, has been expressed in a yeast vector and subjected to structure determination in solution usingExpand
Orientation dependence in fluorescent energy transfer between Cy3 and Cy5 terminally attached to double-stranded nucleic acids
We have found that the efficiency of fluorescence resonance energy transfer between Cy3 and Cy5 terminally attached to the 5′ ends of a DNA duplex is significantly affected by the relativeExpand
Protein kinase substrate recognition studied using the recombinant catalytic domain of AMP-activated protein kinase and a model substrate.
One of the most detailed studies of the interaction between a kinase and its substrate achieved to date is presented, based on modelling rather than a determined structure, for the binding of the ACC1 sequence to the kinase domain. Expand
Structure, folding and activity of the VS ribozyme: importance of the 2‐3‐6 helical junction
It is proposed that the 2‐3‐6 junction organizes important aspects of the structure of the ribozyme to facilitate productive association with the substrate, and it is suggested that this results in an interaction between the substrate and the A730 loop to create the active complex. Expand
Elucidation of the primary and three-dimensional structure of the uterotonic polypeptide kalata B1.
The amino acid sequence and structure of a uterotonic polypeptide extracted from the African plant Oldenlandia affinis DC have been determined and the most likely disulfide connectivity is predicted to be 5-22, 13-27, and 17-29. Expand