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The SLC16 gene family—from monocarboxylate transporters (MCTs) to aromatic amino acid transporters and beyond
The monocarboxylate cotransporter (MCT) family now comprises 14 members, of which only the first four (MCT1–MCT4) have been demonstrated experimentally to catalyse the proton-linked transport ofExpand
Characterisation of human monocarboxylate transporter 4 substantiates its role in lactic acid efflux from skeletal muscle
The characterisation of MCT4 expressed in Xenopus oocytes shows that the protein was correctly targeted to the plasma membrane and rates of substrate transport were determined from the rate of intracellular acidification monitored with the pH‐sensitive dye 2′,7′‐bis‐( carboxyethyl)‐5(6)‐carboxyfluorescein (BCECF). Expand
Human metabolic individuality in biomedical and pharmaceutical research
A comprehensive analysis of genotype-dependent metabolic phenotypes using a genome-wide association study with non-targeted metabolomics to identify genetic loci associated with blood metabolite concentrations and generates many new hypotheses for biomedical and pharmaceutical research. Expand
Basigin (CD147) Is the Target for Organomercurial Inhibition of Monocarboxylate Transporter Isoforms 1 and 4
It is demonstrated by site-directed mutagenesis that removal of all accessible cysteine residues on MCT4 does not prevent this inhibition by pCMBS, and ancillary proteins are required to maintain the catalytic activity of MCTs as well as for their translocation to the plasma membrane. Expand
An energy supply network of nutrient absorption coordinated by calcium and T1R taste receptors in rat small intestine
T1R taste receptors are present throughout the gastrointestinal tract. Glucose absorption comprises active absorption via SGLT1 and facilitated absorption via GLUT2 in the apical membrane.Expand
Fluorescence Resonance Energy Transfer Studies on the Interaction between the Lactate Transporter MCT1 and CD147 Provide Information on the Topology and Stoichiometry of the Complex in Situ *
Controls including photobleaching were employed to confirm that where FRET was observed, it was not an artifact of direct excitation of YFP by the CFP excitation laser, and it was also shown that nonspecific overcrowding of proteins did not induce FRET. Expand
PAT-related amino acid transporters regulate growth via a novel mechanism that does not require bulk transport of amino acids
In Drosophila, two molecules related to mammalian proton-assisted SLC36 amino acid transporters (PATs), CG3424 and CG1139, are potent mediators of growth and are named pathetic (path) for their role in maintaining growth. Expand
Studies on the DIDS-binding Site of Monocarboxylate Transporter 1 Suggest a Homology Model of the Open Conformation and a Plausible Translocation Cycle*
A possible structure for the outward facing (open) conformation of MCT1 is modeled by employing modest rotations of the C-terminal domain of the inner facing conformation modeled previously and has a DIDS-binding site consistent with experimental data and locates Lys38 in a hydrophobic environment at the bottom of a substrate-binding channel. Expand
The SLC16 monocaboxylate transporter family.
The MCT proteins have the typical twelve transmembrane-spanning domain (TMD) topology of membrane transporter proteins, and their structure-function relationship is discussed, especially in relation to the future impact of the single nucleotide polymorphism (SNP) databases and, given their ability to transport pharmacologically relevant compounds, the potential impact for pharmacogenomics. Expand
SLC36A4 (hPAT4) Is a High Affinity Amino Acid Transporter When Expressed in Xenopus laevis Oocytes*
This characterization of hPAT4 as a very high affinity/low capacity non-proton-coupled amino acid transporter raises questions about its physiological role, especially as the transport characteristics of h PAT4 are very similar to the Drosophila orthologue PATH, an amino acid “transceptor” that plays a role in nutrient sensing. Expand