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Perturbation of Anion Balance during Inhibition of Growth of Escherichia coli by Weak Acids
TLDR
Recovery of the normal cytoplasmic pH after the removal of acetate was dependent on the synthesis of glutamate, and the change in the glutamate pool compensated for only a small fraction of the accumulated acetate anion.
Inhibition of Escherichia coli growth by acetic acid: a problem with methionine biosynthesis and homocysteine toxicity.
TLDR
The mechanism by which methionine relieves the growth inhibition of Escherichia coli K-12 that is caused by organic weak acid food preservatives was investigated and it was revealed that the homocysteine pool is increased dramatically in acetate-treated cells, suggesting that acetate inhibits a biosynthetic step downstream from this intermediate.
The role of peptide metabolism in the growth of Listeria monocytogenes ATCC 23074 at high osmolarity.
TLDR
The growth of Listeria monocytogenes ATCC 23074 in defined medium is sensitive to high osmolarity when compared with its growth in complex media, such as brain heart infusion (BHI), and the availability in BHI of the osmoprotectant glycine betaine and peptides are the major contributors.
Potassium channel activation by glutathione‐S‐conjugates in Escherichia coli: protection against methylglyoxal is mediated by cytoplasmic acidification
TLDR
It is demonstrated that mutants lacking the channels can be protected against the lethal effects of methylglyoxal by acidification of the cytoplasm with a weak acid and the implications for pHi‐mediated resistance to methylglyxal are discussed.
Survival during exposure to the electrophilic reagent N-ethylmaleimide in Escherichia coli: role of KefB and KefC potassium channels
TLDR
The role of the KefB and KefC potassium efflux systems in protecting Escherichia coli cells against the toxic effects of the electrophile N-ethylmaleimide has been investigated and the kinetics of the intracellular pH change are crucial to the retention of viability of cells exposed to NEM.
Analysis of the kefA2 mutation suggests that KefA is a cation‐specific channel involved in osmotic adaptation in Escherichia coli
TLDR
The cloning of the kefA gene, the organization of the KefA protein and the phenotype of a missense mutation, keFA, which affects the KfA mechanosensitive channel are described, which shows increased sensitivity to acid hypo‐osmotic shock and data are consistent with abnormal regulation of Kef a in the presence of high K+ concentrations and either betaine or proline.
Rapid inactivation of the Escherichia coli Kdp K+ uptake system by high potassium concentrations
TLDR
Analysis of the expression of the Kdp system in a Kdp+/kdpFABC–lacZ strain shows that levels of K+ that are sufficient to inhibit Kdp activity also repress expression, and growth inhibition of strains solely possessing Kdp arises jointly from inhibition of KDP activity and repression of Kdp gene expression.
Activation of potassium channels during metabolite detoxification in Escherichia coli
TLDR
It is demonstrated that in E. coli cells activation of the KefB efflux system is essential for the survival of exposure to methylglyoxal and this establishes an essential physiological function for theKefB system.
Glutathione-Dependent Conversion ofN-Ethylmaleimide to the Maleamic Acid by Escherichia coli: an Intracellular Detoxification Process
TLDR
It is demonstrated that NEM andN-phenylmaleimide (NPM) are rapidly detoxified by E. coli, independent of new protein synthesis and NAD+-dependent dehydrogenase activity and entirely dependent upon glutathione.
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