Author pages are created from data sourced from our academic publisher partnerships and public sources.
- Publications
- Influence
Laboratory evolution of high-redox potential laccases.
- D. Mate, C. Garcia-Burgos, E. García-Ruiz, A. Ballesteros, S. Camarero, M. Alcalde
- Medicine, Biology
- Chemistry & biology
- 24 September 2010
Thermostable laccases with a high-redox potential have been engineered through a strategy that combines directed evolution with rational approaches. The original laccase signal sequence was replaced… Expand
Laccase engineering: from rational design to directed evolution.
- D. Mate, M. Alcalde
- Biology, Medicine
- Biotechnology advances
- 2015
Laccases are multicopper oxidoreductases considered by many in the biotechonology field as the ultimate "green catalysts". This is mainly due to their broad substrate specificity and relative… Expand
Laccase: a multi‐purpose biocatalyst at the forefront of biotechnology
- D. Mate, M. Alcalde
- Chemistry, Medicine
- Microbial biotechnology
- 3 October 2016
Laccases are multicopper containing enzymes capable of performing one electron oxidation of a broad range of substrates. Using molecular oxygen as the final electron acceptor, they release only water… Expand
Evolving thermostability in mutant libraries of ligninolytic oxidoreductases expressed in yeast
- Eva García-Ruiz, D. Mate, A. Ballesteros, A. Martínez, M. Alcalde
- Biology, Medicine
- Microbial cell factories
- 18 March 2010
BackgroundIn the picture of a laboratory evolution experiment, to improve the thermostability whilst maintaining the activity requires of suitable procedures to generate diversity in combination with… Expand
Functional expression of a blood tolerant laccase in Pichia pastoris
- D. Mate, D. González-Pérez, R. Kittl, R. Ludwig, M. Alcalde
- Biology, Medicine
- BMC Biotechnology
- 30 April 2013
BackgroundBasidiomycete high-redox potential laccases (HRPLs) working in human physiological fluids (pH 7.4, 150 mM NaCl) arise great interest in the engineering of 3D-nanobiodevices for biomedical… Expand
Self-Powered Wireless Carbohydrate/Oxygen Sensitive Biodevice Based on Radio Signal Transmission
- M. Falk, M. Alcalde, +25 authors S. Shleev
- Materials Science, Medicine
- PloS one
- 13 October 2014
Here for the first time, we detail self-contained (wireless and self-powered) biodevices with wireless signal transmission. Specifically, we demonstrate the operation of self-sustained carbohydrate… Expand
Development of chimeric laccases by directed evolution
- I. Pardo, A. I. Vicente, D. Mate, M. Alcalde, S. Camarero
- Biology, Medicine
- Biotechnology and bioengineering
- 1 December 2012
DNA recombination methods are useful tools to generate diversity in directed evolution protein engineering studies. We have designed an array of chimeric laccases with high‐redox potential by in… Expand
Blood tolerant laccase by directed evolution.
- D. Mate, D. González-Pérez, +6 authors M. Alcalde
- Biology, Medicine
- Chemistry & biology
- 21 February 2013
High-redox potential laccases are powerful biocatalysts with a wide range of applications in biotechnology. We have converted a thermostable laccase from a white-rot fungus into a blood tolerant… Expand
Tandem-yeast expression system for engineering and producing unspecific peroxygenase.
- P. Molina-Espeja, Su Ma, D. Mate, R. Ludwig, M. Alcalde
- Biology, Medicine
- Enzyme and microbial technology
- 1 June 2015
Unspecific peroxygenase (UPO) is a highly efficient biocatalyst with a peroxide dependent monooxygenase activity and many biotechnological applications, but the absence of suitable heterologous… Expand
Directed Evolution of Fungal Laccases
- D. Mate, Eva García-Ruiz, S. Camarero, M. Alcalde
- Biology, Medicine
- Current genomics
- 31 March 2011
Fungal laccases are generalists biocatalysts with potential applications that range from bioremediation to novel green processes. Fuelled by molecular oxygen, these enzymes can act on dozens of… Expand