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( R )-Citramalate Synthase in Methanogenic Archaea
TLDR
The Methanococcus jannaschii gene MJ1392 was cloned, and its protein product was hyperexpressed in Escherichia coli and shown to catalyze the condensation of pyruvate and acetyl coenzyme A, with the formation of (R)-citramalate.
Alpha-keto acid chain elongation reactions involved in the biosynthesis of coenzyme B (7-mercaptoheptanoyl threonine phosphate) in methanogenic Archaea.
TLDR
The protein product of the Methanococcus jannaschii MJ0503 gene aksA (AksA) was found to catalyze the condensation of alpha-ketoglutarate and acetylCoA to form trans-homoaconitate, a precursor to coenzyme B and biotin.
Identification of Enzymes Homologous to Isocitrate Dehydrogenase That Are Involved in Coenzyme B and Leucine Biosynthesis in Methanoarchaea
TLDR
Two putative Methanococcus jannaschii isocitrate dehydrogenase genes, MJ1596 and MJ0720, were cloned and overexpressed in Escherichia coli and their gene products were tested for the ability to catalyze the NAD- and NADP-dependent oxidative decarboxylation of intermediates in the biosynthesis of methanoarchaeal coenzyme B.
D-erythro-neopterin biosynthesis in the methanogenic archaea Methanococcus thermophila and Methanobacterium thermoautotrophicum deltaH
The steps in the biosynthetic transformation of GTP to 7,8-dihydro-D-erythro-neopterin (H2neopterin), the precursor to the modified folates found in the methanogenic archaea, has been elucidated for
THE COMPONENT FATTY ACIDS FOUND IN SCLEROTIA OF SCLEROTIUM ROLFSII.