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Sharpening high resolution information in single particle electron cryomicroscopy.
TLDR
EM-BFACTOR is a program that has been designed to widely facilitate the use of the novel method for objective B-factor determination and contrast restoration introduced by Rosenthal and Henderson, concluding that it helps to unravel the high resolution molecular features concealed in experimental density maps, thereby making them better suited for interpretation. Expand
Insights into Minor Group Rhinovirus Uncoating: The X-ray Structure of the HRV2 Empty Capsid
TLDR
The structural comparison between native and empty HRV2 particles unveils a number of pH-sensitive amino acid residues, conserved in rhinoviruses, which participate in the structural rearrangements involved in the uncoating process. Expand
Neutralizing antibodies against the preactive form of respiratory syncytial virus fusion protein offer unique possibilities for clinical intervention
TLDR
A unique class of antibodies specific for the prefusion form of this protein that account for most of the neutralizing activity of either a rabbit serum raised against a vaccinia virus recombinant expressing hRSV_F or a human Ig preparation (Respigam), which was used for prophylaxis before Palivizumab. Expand
Infectious bursal disease virus is an icosahedral polyploid dsRNA virus
TLDR
The results show an unprecedented replicative strategy for dsRNA viruses and suggest that birnaviruses are living viral entities encompassing numerous functional and structural characteristics of positive and negative ssRNA viruses. Expand
The C-terminal domain of the pVP2 precursor is essential for the interaction between VP2 and VP3, the capsid polypeptides of infectious bursal disease virus.
TLDR
It is shown that coexpression of the pVP2 and VP3 polypeptides from independent genes results in the assembly of virus-like particles (VLPs), and that this process does not require the presence of the precursor polyprotein. Expand
Infectious Bursal Disease Virus: Ribonucleoprotein Complexes of a Double-Stranded RNA Virus
TLDR
This work used electron microscopy and functional analysis to characterize these RNP complexes of infectious bursal disease virus, the best characterized member of the Birnaviridae family, and developed a method to purify RNP and VPg–dsRNA complexes. Expand
Uncoating of common cold virus is preceded by RNA switching as determined by X-ray and cryo-EM analyses of the subviral A-particle
TLDR
Structural alterations that take place in the virus architecture during infection are identified using X-ray and cryo-EM analyses and suggest that the RNA–protein interactions prepare RNA and facilitate its subsequent egress via a well-ordered mechanism. Expand
Structural polymorphism of the major capsid protein of a double-stranded RNA virus: an amphipathic alpha helix as a molecular switch.
TLDR
The molecular basis of the conformational flexibility of the major capsid protein precursor, pVP2, is an amphipatic alpha helix formed by the sequence GFKDIIRAIR, which is responsible for the inherent structural polymorphism of VP2. Expand
Infectious Bursal Disease Virus Capsid Assembly and Maturation by Structural Rearrangements of a Transient Molecular Switch
TLDR
Three-dimensional cryo-electron microscopy maps at or close to subnanometer resolution with atomic models suggest that the key polypeptide element, the C-terminal amphipathic α-helix, which acts as a transient conformational switch, is bound to the flexible VP2 C- terminal end. Expand
Epitope Insertion at the N-Terminal Molecular Switch of the Rabbit Hemorrhagic Disease Virus T=3 Capsid Protein Leads to Larger T=4 Capsids
TLDR
The calicivirus capsid protein, and perhaps that of many other viruses, thus can encode polymorphism modulators that are not anticipated from the plane sequence, with important implications for understanding virus assembly and evolution. Expand
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