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Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1
Methylation Mediation Methylation of cytosine bases, 5-methylcytosine (5mC), in DNA plays an important regulatory role in mammalian genomes. Methylation patterns are often inherited acrossExpand
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Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage
Current genome-editing technologies introduce double-stranded (ds) DNA breaks at a target locus as the first step to gene correction. Although most genetic diseases arise from point mutations,Expand
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Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
The spontaneous deamination of cytosine is a major source of transitions from C•G to T•A base pairs, which account for half of known pathogenic point mutations in humans. The ability to efficientlyExpand
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Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage
The spontaneous deamination of cytosine is a major source of transitions from C•G to T•A base pairs, which account for half of known pathogenic point mutations in humans. The ability to efficientlyExpand
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High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity
The RNA-programmable Cas9 endonuclease cleaves double-stranded DNA at sites complementary to a 20-base-pair guide RNA. The Cas9 system has been used to modify genomes in multiple cells and organisms,Expand
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Search-and-replace genome editing without double-strand breaks or donor DNA
Most genetic variants that contribute to disease1 are challenging to correct efficiently and without excess byproducts2–5. Here we describe prime editing, a versatile and precise genome editingExpand
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Evolved Cas9 variants with broad PAM compatibility and high DNA specificity
A key limitation of the use of the CRISPR–Cas9 system for genome editing and other applications is the requirement that a protospacer adjacent motif (PAM) be present at the target site. For the mostExpand
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Opposing Effects of Tcf3 and Tcf1 Control Wnt-Stimulation of Embryonic Stem Cell Self Renewal
The co-occupancy of Tcf3 with Oct4, Sox2 and Nanog on embryonic stem cell (ESC) chromatin indicated that Tcf3 has been suggested to play an integral role in a poorly understood mechanism underlyingExpand
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Fusion of catalytically inactive Cas9 to FokI nuclease improves the specificity of genome modification
Genome editing by Cas9, which cleaves double-stranded DNA at a sequence programmed by a short single-guide RNA (sgRNA), can result in off-target DNA modification that may be detrimental in someExpand
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Increasing the genome-targeting scope and precision of base editing with engineered Cas9-cytidine deaminase fusions
Base editing induces single-nucleotide changes in the DNA of living cells using a fusion protein containing a catalytically defective Streptococcus pyogenes Cas9, a cytidine deaminase, and anExpand
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