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A DNA barcode for land plants
- P. Hollingsworth, L. Forrest, D. Little
- BiologyProceedings of the National Academy of Sciences
- 4 August 2009
The 2-locus combination of rbcL+matK will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.
Choosing and Using a Plant DNA Barcode
The process of selecting and refining a plant barcode is reviewed; the factors which influence the discriminatory power of the approach are evaluated; some early applications of plant barcoding are described and summarise major emerging projects; and outline tool development that will be necessary for plant DNA barcode to advance.
Recent Synchronous Radiation of a Living Fossil
- N. Nagalingum, C. Marshall, T. Quental, H. Rai, D. Little, S. Mathews
- Geography, Environmental ScienceScience
- 11 November 2011
It is shown that cycads underwent a near synchronous global rediversification beginning in the late Miocene, followed by a slowdown toward the Recent, rejecting the hypothesized role of dinosaurs in generating extant diversity and the designation of today’s cycad species as living fossils.
DNA Barcoding in the Cycadales: Testing the Potential of Proposed Barcoding Markers for Species Identification of Cycads
A workflow for DNA barcoding is suggested, including database generation and management, which will ultimately be necessary if the authors are to succeed in establishing a universal DNA barcode for plants.
Evolution and Circumscription of the True Cypresses (Cupressaceae: Cupressus)
- D. Little
- 1 July 2006
Data from anatomy, biochemistry, micromorphology, reproductive development, reproductive morphology, and vegetative morphology were combined with molecular sequence data to produce the most complete hypothesis of evolutionary relationships within Cupressoideae to date.
Phylogeographic relationships among Asian eggplants and new perspectives on eggplant domestication.
A comparison of algorithms for the identification of specimens using DNA barcodes: examples from gymnosperms
- D. Little, D. Stevenson
- Biology, Computer ScienceCladistics : the international journal of the…
- 1 February 2007
Two novel alignment‐free algorithmic solutions (DOME ID and ATIM) are offered to identify query sequences for the purposes of DNA barcoding and application of DNABarcoding to sequences of CITES listed cycads provides an example of the potential application of science to enforcement of conservation laws.
A Functional Phylogenomic View of the Seed Plants
A novel functional phylogenomic approach that explores the genomic origins of seed plant diversification and takes advantage of genomic data to define orthologs, reconstruct relationships, and narrow down candidate genes involved in plant evolution within a phylogenomic view of species' diversification.
Nuclear genome size in Selaginella.
Estimates of nuclear genome size for 9 Selaginella species were obtained using flow cytometry, and measurements for 7 of these species are reported for the first time, and Nuclear genome size appears to be strongly correlated with chromosome number.
DNA Barcode Sequence Identification Incorporating Taxonomic Hierarchy and within Taxon Variability
- D. Little
- BiologyPloS one
- 16 August 2011
A novel alignment–free sequence identification algorithm–BRONX–that accounts for observed within taxon variability and hierarchic relationships among taxa and performs better than all other methods when there is imperfect overlap between query and reference sequences.