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Molecular identification of three distinct Polerovirus species and a recombinant Cucurbit aphid-borne yellows virus strain infecting cucurbit crops in Taiwan
RT-PCR protocols based on sets of specific primers were developed to distinguish between the different cucurbit-infecting polerovirus species and the two CABYV strains, and are discussed in relation to strategies for breeding for durable resistance againstPoleroviruses. Expand
The complete nucleotide sequence of a capsicum chlorosis virus isolate from Lycopersicum esculentum in Thailand
The complete nucleotide sequence of a tospovirus isolated from Lycopersicum esculentum in Thailand was determined and it is proposed to refer to all these different viruses as isolates of CaCV. Expand
Ceratothripoides claratris, a New Vector of a Capsicum chlorosis virus Isolate Infecting Tomato in Thailand.
Transmission efficiency did not significantly differ between adult males and females, irrespective of the larval stage at which the virus was acquired, and the spread of CaCV on tomato plants in greenhouses showed a close association with thrips infestations. Expand
First report of Tomato brown rugose fruit virus infecting tomato in Germany
In July 2018, unusual fruit and leaf symptoms were observed in numerous greenhouses cultivating tomatoes commercially in the Lower Rhine region of North-Rhine Westphalia, Germany. Foliar symptomsExpand
Identification of a novel nanovirus in parsley
The genome sequence of the virus shows that it is a member of the genus Nanovirus, but it lacks DNA-U4, and the name “parsley severe stunt associated virus” (PSSaV) is proposed for this novel nanovirus. Expand
First report of maize yellow mosaic virus infecting maize (Zea mays) in Ethiopia
This first report of MaYMV in Ethiopia provides evidence for its nationwide distribution and the prevalence of a virus that may have occurred in Ethiopia for some time but was overlooked in targeted surveys focusing on MLND and other prominent maize diseases. Expand
Application of Phi29 DNA polymerase in identification and full-length clone inoculation of tomato yellow leaf curl Thailand virus and tobacco leaf curl Thailand virus
Summary.Tomato plants grown in greenhouses in Thailand developed typical symptoms of a tomato yellow leaf curl Thailand virus (TYLCTHV) infection. After confirmation by ELISA, a Phi29 DNA polymeraseExpand
Immunocapture of virions with virus-specific antibodies prior to high-throughput sequencing effectively enriches for virus-specific sequences
The first complete genome sequence of a potato yellowing virus isolate (PYV, DSMZ PV-0706) was determined and can be confidently assigned as a distinct species in the genus Ilarvirus. Expand
Full‐length genome sequences of four polerovirus isolates infecting cucurbits in Taiwan determined from total RNA extracted from field samples
TW19 represents the first full-length sequence for the recently described Suakwa aphid-borne yellows virus (SABYV), and the deduced aa sequences for all the proteins of TW19 showed >10% variation compared to all other poleroviruses, though recombination analysis suggested MABYV as the major parent and an unidentifiedPolerovirus as minor parent. Expand
Molecular diversity of poleroviruses infecting cucurbit crops in four countries reveals the presence of members of six distinct species
C cucurbit samples with yellowing symptoms from fields in Mali, the Philippines, Thailand and Uzbekistan were screened by RT-PCR using universal polerovirus primers, and the presence of two polerOViruses that had not been described previously were revealed. Expand