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Guidelines for the use and interpretation of assays for monitoring autophagy
These guidelines are presented for the selection and interpretation of methods for use by investigators who aim to examine macroautophagy and related processes, as well as for reviewers who need to provide realistic and reasonable critiques of papers that are focused on these processes.
Autophagy fights disease through cellular self-digestion
Understanding autophagy may ultimately allow scientists and clinicians to harness this process for the purpose of improving human health, and to play a role in cell death.
Regulation mechanisms and signaling pathways of autophagy.
The current knowledge on the key genes composing the autophagy machinery in eukaryotes from yeast to mammalian cells and the signaling pathways that sense the status of different types of stress and induce autophagic for cell survival and homeostasis are presented.
Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes
A set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes are presented.
Development by self-digestion: molecular mechanisms and biological functions of autophagy.
Autophagosome formation: core machinery and adaptations
A core molecular machinery has a critical role in forming sequestering vesicles, the autophagosome, which is the hallmark morphological feature of this dynamic process.
Mammalian autophagy: core molecular machinery and signaling regulation.
Autophagy as a regulated pathway of cellular degradation.
The core protein machinery that is necessary to drive formation and consumption of intermediates in the macroautophagy pathway includes a ubiquitin-like protein conjugation system and a protein complex that directs membrane docking and fusion at the lysosome or vacuole.
A protein conjugation system essential for autophagy
It is shown here that a unique covalent-modification system is essential for autophagy to occur, the first report of a protein unrelated to ubiquitin that uses a ubiquitination-like conjugation system.
Autophagy: from phenomenology to molecular understanding in less than a decade
- D. Klionsky
- BiologyNature Reviews Molecular Cell Biology
- 1 November 2007
In 2000, it was suggested to me that “Autophagy will be the wave of the future; it will become the new apoptosis,” and this statement turned out to be prophetic, and this process of 'self-eating' rapidly exploded as a research field, as scientists discovered connections to cancer, neurodegeneration and even lifespan extension.