Efficient recovery of environmental DNA for expression cloning by indirect extraction methods.
- E. Gabor, E. de Vries, D. Janssen
- BiologyFEMS Microbiology Ecology
- 1 May 2003
Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10
- S. Keuning, D. Janssen, B. Witholt
- Biology, ChemistryJournal of Bacteriology
- 1 August 1985
A new enzyme, haloalkane dehalogenase, was isolated from the 1,2-dichloroethane-utilizing bacterium Xanthobacter autotrophicus GJ10. The purified enzyme catalyzed the hydrolytic dehalogenation of…
Quantifying the accessibility of the metagenome by random expression cloning techniques.
- E. Gabor, W. Alkema, D. Janssen
- BiologyEnvironmental Microbiology
- 1 September 2004
This study reveals significant differences in the predicted expression modes between distinct taxonomic groups of organisms and suggests that about 40% of the enzymatic activities may be readily recovered by random cloning in E. coli.
Identification of a Baeyer–Villiger monooxygenase sequence motif
- M. Fraaije, Nanne M. Kamerbeek, W. V. van Berkel, D. Janssen
- BiologyFEBS Letters
- 8 May 2002
Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10
- D. Janssen, A. Scheper, L. Dijkhuizen, B. Witholt
- ChemistryApplied and Environmental Microbiology
- 1 March 1985
This article corrects the article on p. 674 in vol.
Baeyer-Villiger monooxygenases, an emerging family of flavin-dependent biocatalysts
- N. Kamerbeek, D. Janssen, W. Berkel, M. Fraaije, N. Kamerbeek, W. V. Berkel
- Biology, Engineering
- 1 June 2003
The biocatalytic properties of recently cloned BV MOs are reviewed and the potential for obtaining novel BVMOs from sequenced genomes will be discussed.
Handbook on Biodegradation and Biological Treatment of Hazardous Organic Compounds
- M. H. V. Agteren, S. Keuning, D. Janssen
- Chemistry, Biology
- 31 May 1998
This chapter discusses polycyclic Aromatic Hydrocarbons, a class of hydrocarbons that combines halogenated Aliphatic Compounds and Chloroaromatic Compounds, and some of the properties of these compounds.
A Glutathione S-Transferase with Activity towards cis-1,2-Dichloroepoxyethane Is Involved in Isoprene Utilization by Rhodococcus sp. Strain AD45
- J. E. van Hylckama Vlieg, J. Kingma, A. J. van den Wijngaard, D. Janssen
- ChemistryApplied and Environmental Microbiology
- 1 August 1998
The results indicate that a GSHS-transferase is involved in the metabolism of isoprene and that the enzyme can detoxify reactive epoxides produced by monooxygenation of chlorinated ethenes.
Cloning of 1,2-dichloroethane degradation genes of Xanthobacter autotrophicus GJ10 and expression and sequencing of the dhlA gene
- D. Janssen, F. Pries, J. R. van der Ploeg, B. Kazemier, P. Terpstra, B. Witholt
- Biology, EngineeringJournal of Bacteriology
- 1 December 1989
A gene bank from the chlorinated hydrocarbon-degrading bacterium Xanthobacter autotrophicus GJ10 was prepared in the broad-host-range cosmid vector pLAFR1 and the haloalkane dehalogenase gene was identified by the known N-terminal amino acid sequence of its product and found to encode a 310-amino-acid protein of molecular weight 35,143.
Characterization of the Gene Cluster Involved in Isoprene Metabolism in Rhodococcus sp. Strain AD45
- J. E. van Hylckama Vlieg, H. Leemhuis, J. H. Spelberg, D. Janssen
- Biology, ChemistryJournal of Bacteriology
- 1 April 2000
The results are in agreement with a catabolic route for isoprene involving epoxidation by a monooxygenase, conjugation to glutathione, and oxidation of the hydroxyl group to a carboxylate.
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