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Efficient recovery of environmental DNA for expression cloning by indirect extraction methods.
It is suggested that the use of indirect DNA isolation methods allows the construction of environmental gene banks of superior quality, as eukaryotic genetic information is generally not expressed in bacterial host organisms but increases the library size. Expand
Purification and characterization of hydrolytic haloalkane dehalogenase from Xanthobacter autotrophicus GJ10.
A new enzyme, haloalkane dehalogenase, was isolated from the 1,2-dichloroethane-utilizing bacterium Xanthobacter autotrophicus GJ10. The purified enzyme catalyzed the hydrolytic dehalogenation of… Expand
Quantifying the accessibility of the metagenome by random expression cloning techniques.
This study reveals significant differences in the predicted expression modes between distinct taxonomic groups of organisms and suggests that about 40% of the enzymatic activities may be readily recovered by random cloning in E. coli. Expand
Handbook on Biodegradation and Biological Treatment of Hazardous Organic Compounds
Preface. 1. Introduction. 2. Aliphatic Compounds. 3. Halogenated Aliphatic Compounds. 4. Aromatic Compounds. 5. Polycyclic Aromatic Hydrocarbons. 6. Chloroaromatic Compounds. 7. Synonyms.… Expand
A Glutathione S-Transferase with Activity towards cis-1,2-Dichloroepoxyethane Is Involved in Isoprene Utilization by Rhodococcus sp. Strain AD45
- J. V. H. van Hylckama Vlieg, J. Kingma, A. J. van den Wijngaard, D. Janssen
- Chemistry, Medicine
- Applied and Environmental Microbiology
- 1 August 1998
The results indicate that a GSHS-transferase is involved in the metabolism of isoprene and that the enzyme can detoxify reactive epoxides produced by monooxygenation of chlorinated ethenes. Expand
Transformation Kinetics of Chlorinated Ethenes by Methylosinus trichosporium OB3b and Detection of Unstable Epoxides by On-Line Gas Chromatography.
- V. J. van Hylckama, W. de Koning, D. Janssen
- Chemistry, Medicine
- Applied and environmental microbiology
- 1 September 1996
A rapid and accurate method for the determination of transformation kinetics of volatile organic substrates was developed and Methane and acetylene inhibited degradation of the epoxide of cis-1,2-dichloroethene, indicating that sMMO was involved. Expand
Identification of a Baeyer–Villiger monooxygenase sequence motif
Using newly characterized BVMO sequences, a BV MO‐identifying sequence motif is uncovered: FXGXXXHXXXW(P/D). Expand
Baeyer-Villiger monooxygenases, an emerging family of flavin-dependent biocatalysts
Baeyer-Villiger monooxygenases (BVMOs) are flavoenzymes that catalyze a remarkably wide variety of oxidative reactions such as regio- and enantioselective Baeyer-Villiger oxidations and… Expand
Degradation of halogenated aliphatic compounds by Xanthobacter autotrophicus GJ10.
The results show that X. autotrophicus may play a role in the degradation of organochlorine compounds and that hydrolytic dehalogenases may be involved in the microbial metabolism of short-chain halogenated hydrocarbons in microorganisms. Expand