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Regulated transport of the glucose transporter GLUT4
In muscle and fat cells, insulin stimulates the delivery of the glucose transporter GLUT4 from an intracellular location to the cell surface, where it facilitates the reduction of plasma glucoseExpand
Immuno-localization of the insulin regulatable glucose transporter in brown adipose tissue of the rat
The results suggest that in the presence of insulin GLUT 4 recycles from the cell surface, probably via the coated pit-endosome pathway that has been characterized for cell surface receptors, and also that insulin causes the redistribution ofGLUT 4 by stimulating exocytosis from GLUT 2-containing tubulo-vesicular structures, rather than by slowing endocytotic of GLUT 3. Expand
Interleukin-6 Increases Insulin-Stimulated Glucose Disposal in Humans and Glucose Uptake and Fatty Acid Oxidation In Vitro via AMP-Activated Protein Kinase
It is demonstrated that acute IL- 6 treatment enhances insulin-stimulated glucose disposal in humans in vivo, while the effects of IL-6 on glucose and fatty acid metabolism in vitro appear to be mediated by AMPK. Expand
Characterization of the Role of the Rab GTPase-activating Protein AS160 in Insulin-regulated GLUT4 Trafficking*
Findings support an important role for AS160 in the insulin regulated trafficking of GLUT4, which is mediated by the cytosolic tail of insulin-regulated aminopeptidase which interacted both in vitro and in vivo with AS160. Expand
Dissecting multiple steps of GLUT4 trafficking and identifying the sites of insulin action.
These data show that Akt-dependent phosphorylation of AS160 is not the major regulated step in GLUT4 trafficking, implicating alternative Akt substrates or alternative signaling pathways downstream of GSV docking at the cell surface as the major regulatory node. Expand
Berberine, a Natural Plant Product, Activates AMP-Activated Protein Kinase With Beneficial Metabolic Effects in Diabetic and Insulin-Resistant States
It is suggested that berberine displays beneficial effects in the treatment of diabetes and obesity at least in part via stimulation of AMPK activity. Expand
Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events
Data show that combinatorial interactions of SNAREs determine whether late endosomes undergo homotypic or heterotypic fusion events, and that the same Q‐SNAREs can combine with an alternative R‐ SNARE, namely VAMP7, for heterotyping fusion between late endOSomes and lysosomes. Expand
Molecular cloning and characterization of an insulin-regulatable glucose transporter
Cl cloning and sequencing of cDNAs isolated from both rat adipocyte and heart libraries that encode a protein recognized by mAb 1F8, and which has 65% sequence identity to the human HepG2 glucose transporter are described, indicating that this cDNA encodes a membrane protein with the characteristics of the translocatable glucose transporter expressed in insulin-responsive tissues. Expand
Flotillin-1/Reggie-2 Traffics to Surface Raft Domains via a Novel Golgi-independent Pathway
It is shown that newly synthesized flotillin-1 reaches the plasma membrane via a Sar1-independent and brefeldin A-resistant targeting pathway, involving a Golgi-independent trafficking pathway, the PHB domain, and palmitoylation. Expand
Structure of the Munc18c/Syntaxin4 N-peptide complex defines universal features of the N-peptide binding mode of Sec1/Munc18 proteins
Results support a sequential two-state model for SM/SNARE binding involving an initial interaction via the Stx N-peptide, which somehow facilitates a second, more comprehensive interaction comprising other contact surfaces in both proteins. Expand