Reductive glutamine metabolism by IDH1 mediates lipogenesis under hypoxia
- C. Metallo, P. Gameiro, G. Stephanopoulos
- 1 November 2011
It is shown that human cells use reductive metabolism of α-ketoglutarate to synthesize AcCoA for lipid synthesis and support lipid synthesis in mammalian cells.
Direct observation of ligand recognition by T cells
- D. Irvine, M. Purbhoo, M. Krogsgaard, Mark M. Davis
- 24 October 2002
It is shown that T cells expressing the CD4 antigen respond with transient calcium signalling to even a single agonist peptide–MHC ligand, and that the organization of molecules in the contact zone of the T cell and APC takes on the characteristics of an immunological synapse when only about ten agonists are present.
TRK RECEPTORS: ROLES IN NEURONAL SIGNAL TRANSDUCTION *
- D. Irvine, Lawren C. Wu, P. B. Moore
- Biology, Chemistry
Perhaps the most fascinating aspect of Trk receptor-mediated signaling is its interplay with signaling promoted by the pan-neurotrophin receptor p75 .
T cell killing does not require the formation of a stable mature immunological synapse
- M. Purbhoo, D. Irvine, J. Huppa, Mark M. Davis
- BiologyNature Immunology
- 1 May 2004
A single-molecule labeling method is used to directly measure the number of ligands a cytotoxic T cell engages and track the consequences of that interaction by three-dimensional video microscopy, showing that stable synapse formation and complete signaling are not required for cytotoxicity.
Structure-based programming of lymph-node targeting in molecular vaccines
- Haipeng Liu, K. Moynihan, D. Irvine
- Biology, ChemistryNature
- 1 February 2014
Administration of structurally optimized CpG-DNA/peptide amph-vaccines in mice resulted in marked increases in LN accumulation and decreased systemic dissemination relative to their parent compounds, leading to 30-fold increases in T-cell priming and enhanced anti-tumour efficacy while greatly reducing systemic toxicity.
Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles.
- A. Verma, O. Uzun, F. Stellacci
- BiologyNature Materials
- 1 July 2008
It is shown that the former particles penetrate the plasma membrane without bilayer disruption, whereas the latter are mostly trapped in endosomes, offering a paradigm for analysing the fundamental problem of cell-membrane-penetrating bio- and macro-molecules.
Therapeutic cell engineering using surface-conjugated synthetic nanoparticles
- M. Stephan, J. Moon, Soong Ho Um, A. Bershteyn, D. Irvine
- BiologyNature Network Boston
- 4 June 2010
With this method of providing sustained pseudoautocrine stimulation to donor cells, marked enhancements in tumor elimination are elicited in a model of adoptive T cell therapy for cancer and suggest therapeutic cells are promising vectors for actively targeted drug delivery.
Nanoparticulate STING agonists are potent lymph node-targeted vaccine adjuvants.
- M. Hanson, M. P. Crespo, D. Irvine
- BiologyJournal of Clinical Investigation
- 1 May 2015
It is demonstrated that nanoparticulate delivery safely targets CDNs to the dLNs and enhances the efficacy of this adjuvant, and this approach can be broadly applied to other small-molecule immunomodulators of interest for vaccines and immunotherapy.
A robust, high-throughput assay to determine the phagocytic activity of clinical antibody samples.
- M. Ackerman, B. Moldt, G. Alter
- BiologyJIM - Journal of Immunological Methods
- 1 December 2010
Interleukin-7 Receptor Signaling Network: An Integrated Systems Perspective
- M. J. Palmer, V. Mahajan, L. C. Trajman, D. Irvine, D. Lauffenburger, Jianzhu Chen
- BiologyCellular & Molecular Immunology
- 1 April 2008
This review examines how the strength of the signal through the IL-7 receptor is modulated in T and B cells, including the use of shared receptor components, signaling crosstalk, shared interaction domains, feedback loops, integrated gene regulation, multimerization and ligand competition.