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Lactobacillus casei microbiological assay of folic acid derivatives in 96-well microtiter plates.
Microbiological assay is still widely used for estimating folic acid derivatives in serum and other biological samples. We describe here a modification of this procedure involving use of 96-well… Expand
Microbiological assay of folates in 96-well microtiter plates.
- D. Horne
- Chemistry, Medicine
- Methods in enzymology
Publisher Summary This chapter presents the microbiological assay of folates in 96-well microtiter plates and outlines the problem inherent to clear-wall plates and turbidimetric assays, which… Expand
High-performance liquid chromatographic determination of the distribution of naturally occurring folic acid derivatives in rat liver.
Procedures which allow extraction and quantitation of labile, reduced folic acid derivatives in rat liver have been developed. These procedures entail extraction of hepatic folates at 100 degrees C… Expand
Apoptosis of late-stage erythroblasts in megaloblastic anemia: association with DNA damage and macrocyte production.
An in vitro model of folate-deficient erythropoiesis has been developed using proerythroblasts isolated from the spleens of Friend virus-infected mice fed an amino acid-based, folate-free diet.… Expand
Apoptosis mediates and thymidine prevents erythroblast destruction in folate deficiency anemia.
Deficiency of the vitamin folic acid causes pancytopenia by decreasing the production of new blood cells. Although impaired DNA synthesis and destruction of hematopoietic cells have been implicated,… Expand
Use of glycerol-cryoprotected Lactobacillus casei for microbiological assay of folic acid.
A simple procedure for preparing glycerol-cryoprotected Lactobacillus casei cultures has been developed. L. casei grown in medium supplemented with low concentrations of folic acid (0.3 micrograms/L)… Expand
Effect of dietary methyl group deficiency on one-carbon metabolism in rats.
Amino acid-defined diets deficient in methyl groups have been shown to result in a very high incidence of hepatocellular carcinoma. It has been suggested that this is a result of decreased levels of… Expand
Metabolic role of cytoplasmic isozymes of 5,10-methylenetetrahydrofolate dehydrogenase in Saccharomyces cerevisiae.
Saccharomyces cerevisiae possesses two cytosolic 5,10-methylenetetrahydrofolate (CH2-THF) dehydrogenases that differ in their redox cofactor specificity: an NAD-dependent dehydrogenase encoded by the… Expand