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The purification and properties of Escherichia coli methylglyoxal synthase.
1. Methylglyoxal synthase was purified over 1500-fold from glycerol-grown Escherichia coli K 12 strain CA 244. The purified enzyme was inactivated by heat or proteolysis, had a molecular weight of… Expand
The regulation of Escherichia coli methylglyoxal synthase; a new control site in glycolysis?
A novel sequence of reactions for the conversion of dihydroxyacetone phosphate (DHAP) to pyruvate was described recently [ 1 ] . These reactions in which methylglyoxal and D-lactate are intermediates… Expand
The purification and characterization of 4-ethylphenol methylenehydroxylase, a flavocytochrome from Pseudomonas putida JD1.
The enzyme 4-ethylphenol methylenehydroxylase was purified from Pseudomonas putida JD1 grown on 4-ethylphenol. It is a flavocytochrome c for which the Mr was found to be 120,000 by ultracentrifuging… Expand
Alkylphenol Biotransformations Catalyzed by 4-Ethylphenol Methylenehydroxylase
ABSTRACT 4-Ethylphenol methylenehydroxylase from Pseudomonas putida JD1 acts by dehydrogenation of its substrate to give a quinone methide, which is then hydrated to an alcohol. It was shown to be… Expand
Gentisic acid and its 3- and 4-methyl-substituted homologoues as intermediates in the bacterial degradation of m-cresol, 3,5-xylenol and 2,5-xylenol.
1. Intact cells of a non-fluorescent Pseudomonas grown with m-cresol, 2,5-xylenol, 3,5-xylenol, 3-ethyl-5-methylphenol or 2,3,5-trimethylphenol rapidly oxidized all these phenols to completion.… Expand
p-Cresol methylhydroxylase. Assay and general properties.
p-Cresol methylhydroxylase from Pseudomonas putida, an anaerobic dehydrogenase that catalyses the oxidation of p-cresol to p-hydroxybenzyl alcohol and then to p-hydroxybenzaldehyde, is an enzyme of… Expand
The purification and properties of p-cresol-(acceptor) oxidoreductase (hydroxylating), a flavocytochrome from Pseudomonas putida.
The enzyme that catalyses the hydroxylation of the methyl group of p-cresol was purified from Pseudomonas putida. It has mol.wt. 115000 and appears to contain two subunits of equal molecular weight.… Expand
The bacterial metabolism of 2,4-xylenol.
1. Measurements of the rates of oxidation of various compounds by a fluorescent Pseudomonas indicated that metabolism of 2,4-xylenol was initiated by oxidation of the methyl group para to the… Expand
The hydroxylation of P-cresol and its conversion to P-hydroxybenzaldehyde in Pseudomonas putida.
- D. Hopper
- Chemistry, Medicine
- Biochemical and biophysical research…
- 22 March 1976
Abstract Cell extracts of Pseudomonas putida catalyze the conversion of p -cresol to p -hydroxybenzylalcohol when phenazine methosulfate, an electron acceptor, is added. The reaction will proceed… Expand
The purification and properties of 4-hydroxyisophthalate hydroxylase from Pseudomonas putida NCIB 9866.
1 4-Hydroxyisophthalate hydroxylase has been purified from cells grown with 2,4-xylenol and gave a single protein band on polyacrylamide-gel electrophoresis after a 24-fold purification. 2 In… Expand