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Large-scale fluid/fluid phase separation of proteins and lipids in giant plasma membrane vesicles
The membrane raft hypothesis postulates the existence of lipid bilayer membrane heterogeneities, or domains, supposed to be important for cellular function, including lateral sorting, signaling, andExpand
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Bimolecular Fluorescence Complementation ( BiFC ) Analysis as a Probe of Protein Interactions in Living Cells
Protein interactions are a fundamental mechanism for the generation of biological regulatory specificity. The study of protein interactions in living cells is of particular significance because theExpand
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Critical fluctuations in plasma membrane vesicles.
We demonstrate critical behavior in giant plasma membrane vesicles (GPMVs) that are isolated directly from living cells. GPMVs contain two liquid phases at low temperatures and one liquid phase atExpand
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Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells
Tumor progression involves the ability of cancer cells to communicate with each other and with neighboring normal cells in their microenvironment. Microvesicles (MV) derived from human cancer cellsExpand
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Crosslinking a lipid raft component triggers liquid ordered-liquid disordered phase separation in model plasma membranes.
The mechanisms by which a cell uses and adapts its functional membrane organization are poorly understood and are the subject of ongoing investigation and discussion. Here, we study one proposedExpand
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A Lipid Raft Environment Enhances Lyn Kinase Activity by Protecting the Active Site Tyrosine from Dephosphorylation*
The plasma membrane contains ordered lipid domains, commonly called lipid rafts, enriched in cholesterol, sphingolipids, and certain signaling proteins. Lipid rafts play a structural role in signalExpand
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Compartmentalized Activation of the High Affinity Immunoglobulin E Receptor within Membrane Domains*
The earliest known step in the activation of the high affinity IgE receptor, FcεRI, is the tyrosine phosphorylation of its β and γ subunits by the Src family tyrosine kinase, Lyn. We report here thatExpand
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Correlation Functions Quantify Super-Resolution Images and Estimate Apparent Clustering Due to Over-Counting
We present an analytical method using correlation functions to quantify clustering in super-resolution fluorescence localization images and electron microscopy images of static surfaces in twoExpand
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Fluorescence anisotropy measurements of lipid order in plasma membranes and lipid rafts from RBL-2H3 mast cells.
Specialized plasma membrane domains known as lipid rafts participate in signal transduction and other cellular processes, and their liquid ordered (L(o)) phase appears to be important for theirExpand
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Structural determinants for partitioning of lipids and proteins between coexisting fluid phases in giant plasma membrane vesicles.
The structural basis for organizational heterogeneity of lipids and proteins underlies fundamental questions about the plasma membrane of eukaryotic cells. A current hypothesis is the participationExpand
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