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Direct quantification of inorganic polyphosphate in microbial cells using 4'-6-diamidino-2-phenylindole (DAPI).
TLDR
A direct fluorescence based DAPI assay system which removes the requirement for prior polyP extraction before quantification, which increased the efficiency of polyP detection by 28-55% in microbial cultures suggesting quantitative measurement of the intracellular polyP pool.