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Detection of Legionella Species in Respiratory Specimens Using PCR with Sequencing Confirmation
ABSTRACT Legionella spp. are a common cause of community-acquired respiratory tract infections and an occasional cause of nosocomial pneumonia. A PCR method for the detection of legionellae inExpand
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Serious bacterial infections in febrile infants 1 to 90 days old with and without viral infections.
OBJECTIVE The risk of serious bacterial infection (SBI) in febrile infants who are classified as low risk (LR) or high risk (HR) by the Rochester criteria has been established. LR infants average aExpand
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Diagnosis and Outcomes of Enterovirus Infections in Young Infants
Background: Enterovirus (EV) infections commonly cause fever in infants younger than 90 days of age. The polymerase chain reaction (PCR) has improved our ability to diagnose EV infections. Objective:Expand
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Large-Scale Analysis of the Prevalence and Geographic Distribution of HIV-1 Non-B Variants in the United States
ABSTRACT The genetic diversity of human immunodeficiency virus type 1 (HIV-1) has significant implications for diagnosis, vaccine development, and clinical management of patients. Although HIV-1Expand
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Automated polymerase chain reaction in capillary tubes with hot air.
We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatusExpand
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Identification of Mycobacterium spp. by Using a Commercial 16S Ribosomal DNA Sequencing Kit and Additional Sequencing Libraries
ABSTRACT Current methods for identification of Mycobacterium spp. rely upon time-consuming phenotypic tests, mycolic acid analysis, and narrow-spectrum nucleic acid probes. Newer approaches includeExpand
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DNA ligase and the pyridine nucleotide cycle in Salmonella typhimurium.
Bacterial DNA ligases use NAD as an energy source. In this study we addressed two questions about these enzymes. First, what is the physiological consequence of completely removing the NAD-dependentExpand
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A polymerase chain reaction-based epidemiologic investigation of the incidence of nonpolio enteroviral infections in febrile and afebrile infants 90 days and younger.
OBJECTIVE Enteroviruses are important pathogens in infants, but their true contribution to febrile illness in infants </=90 days old is unknown. The purpose of this study was to use the polymeraseExpand
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Rapid detection of the staphylococcal mecA gene from BACTEC blood culture bottles by the polymerase chain reaction.
A rapid polymerase chain reaction (PCR) method for the direct detection of the staphylococcal mecA gene from BACTEC blood culture bottles (Becton Dickinson, Sparks, MD) was developed. PublishedExpand
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Human Immunodeficiency Virus Type 1 Drug Resistance Testing: a Comparison of Three Sequence-Based Methods
ABSTRACT The use of genotypic assays for determining drug resistance in human immunodeficiency virus (HIV) type 1 (HIV-1)-infected patients is increasing. These tests lack standardization andExpand
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