• Publications
  • Influence
Histone methylation by PRC2 is inhibited by active chromatin marks.
TLDR
It is found that H3K4me3 inhibits PRC2 activity in an allosteric fashion assisted by the Su(z)12 C terminus, which provides the molecular basis of histone H3 N terminus recognition by thePRC2 Nurf55-Su( z)12 submodule.
HDAC‐6 interacts with and deacetylates tubulin and microtubules in vivo
TLDR
The data provide evidence that HDAC‐6 might act as a dual deacetylase for tubulin and histones, and suggest the possibility that acetylated non‐histone proteins might represent novel targets for pharmacological therapy by HDAC inhibitors.
Molecular mechanism for the regulation of protein kinase B/Akt by hydrophobic motif phosphorylation.
TLDR
Analysis of the crystal structures of the unphosphorylated and Thr 309 phosphorylated states of the PKB kinase domain provides a molecular explanation for regulation by Ser 474 phosphorylation.
Identification of a PKB/Akt Hydrophobic Motif Ser-473 Kinase as DNA-dependent Protein Kinase*♦
TLDR
It is concluded that DNA-PK is a long sought after kinase responsible for the Ser-473 phosphorylation step in the activation of PKB.
Stabilization of Mdm2 via Decreased Ubiquitination Is Mediated by Protein Kinase B/Akt-dependent Phosphorylation*
TLDR
It is shown that PKB inhibits Mdm2 self-ubiquitination via phosphorylation of MDM2 on Ser166 and Ser188, and activation of PKB correlated with Mdm3 stability in a variety of human tumor cells, suggesting that P KB plays a critical role in controlling of the Mdn2·p53 signaling pathway by regulating Mdm1 stability.
C-Mannosylation and O-Fucosylation of the Thrombospondin Type 1 Module*
TLDR
By using a novel mass spectrometric approach, it was found that Ser-377, Thr-432, and Thr-489 in the motif CSX(S/T)CG carry the O-linked disaccharide Glc-Fuc-O-Ser/Thr, the first protein in which such a disacCharide has been identified, although proteinO-fucosylation is well described in epidermal growth factor-like modules.
The HRPT2 Tumor Suppressor Gene Product Parafibromin Associates with Human PAF1 and RNA Polymerase II
TLDR
It is shown that parafibromin physically interacts with human orthologs of yeast Paf1 complex components, including PAF1, LEO1, and CTR9, that are involved in transcription elongation and 3′ end processing and RNA processing machineries during the transcription cycle, and RNAi-induced downregulation of parafabromin promotes entry into S phase, implying a role for parafIBromin as an inhibitor of cell cycle progression.
Control of Nutrient-Sensitive Transcription Programs by the Unconventional Prefoldin URI
TLDR
Functional analysis of the yeast and human orthologs of URI revealed that both are targets of nutrient signaling and participate in gene expression controlled by the TOR kinase, suggesting that URI is a component of a signaling pathway that coordinates nutrient availability with gene expression.
Phosphorylation of IQGAP1 Modulates Its Binding to Cdc42, Revealing a New Type of Rho-GTPase Regulator*
TLDR
It is proposed that, depending on its phosphorylation state, IQGAP1 might serve as an effector or sequester nucleotide-free Cdc42 to prevent signaling and uncovers a novel type of Rho-GTPase regulator.
Crystal structure of the human COP9 signalosome
TLDR
The crystal structure of the entire ∼350-kDa human CSN holoenzyme is presented, detailing the molecular architecture of the complex, and it is found that neddylated CRL binding to CSN is sensed by CSN4, and communicated toCSN5 with the assistance of CSN6, resulting in activation of the deneddylase.
...
1
2
3
4
5
...