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Role of SRP RNA in the GTPase cycles of Ffh and FtsY.
The bacterial homologues of the signal recognition particle (SRP) and its receptor, the Ffh*4.5S RNA ribonucleoprotein complex and the FtsY protein, respectively, form a unique complex in which both… Expand
Catalytic promiscuity and the evolution of new enzymatic activities.
Several contemporary enzymes catalyze alternative reactions distinct from their normal biological reactions. In some cases the alternative reaction is similar to a reaction that is efficiently… Expand
Direct Measurement of the Full, Sequence-Dependent Folding Landscape of a Nucleic Acid
- M. Woodside, P. Anthony, William M. Behnke-Parks, Kevan Larizadeh, D. Herschlag, S. Block
- Medicine, Chemistry
- 10 November 2006
Nucleic acid hairpins provide a powerful model system for understanding macromolecular folding, with free-energy landscapes that can be readily manipulated by changing the hairpin sequence. The full… Expand
The DEAD box protein eIF4A. 1. A minimal kinetic and thermodynamic framework reveals coupled binding of RNA and nucleotide.
eIF4A is the archetypal member of the DEAD box family of proteins and has been proposed to use the energy from ATP hydrolysis to unwind structures in the 5'-untranslated regions of eukaryotic mRNAs… Expand
Structural and functional comparisons of nucleotide pyrophosphatase/phosphodiesterase and alkaline phosphatase: implications for mechanism and evolution.
The rapid expansion of the amount of genomic and structural data has provided many examples of enzymes with evolutionarily related active sites that catalyze different reactions. Functional… Expand
Mechanistic aspects of enzymatic catalysis: lessons from comparison of RNA and protein enzymes.
A classic approach in biology, both organismal and cellular, is to compare morphologies in order to glean structural and functional commonalities. The comparative approach has also proven valuable on… Expand
Kinetic dissection of fundamental processes of eukaryotic translation initiation in vitro
Approaches have been developed for the kinetic dissection of eukaryotic translation initiation in vitro using rabbit reticulocyte ribosomes and a crude preparation of initiation factors. These new… Expand
Comparative enzymology in the alkaline phosphatase superfamily to determine the catalytic role of an active-site metal ion.
Mechanistic models for biochemical systems are frequently proposed from structural data. Site-directed mutagenesis can be used to test the importance of proposed functional sites, but these data do… Expand
Catalysis of RNA cleavage by the Tetrahymena thermophila ribozyme. 2. Kinetic description of the reaction of an RNA substrate that forms a mismatch at the active site.
The site-specific endonuclease reaction catalyzed by the ribozyme from the Tetrahymena pre-rRNA intervening sequence has been characterized with a substrate that forms a "matched" duplex with the 5'… Expand
Ras-catalyzed hydrolysis of GTP: a new perspective from model studies.
- K. A. Maegley, S. J. Admiraal, D. Herschlag
- Chemistry, Medicine
- Proceedings of the National Academy of Sciences…
- 6 August 1996
Despite the biological and medical importance of signal transduction via Ras proteins and despite considerable kinetic and structural studies of wild-type and mutant Ras proteins, the mechanism of… Expand